Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human Models.

Mehdi Djelloul; Staffan Holmqvist; Antonio Boza Serrano; Carla Azevedo; Maggie S Yeung; Stefano Goldwurm; Jonas Frisén; Tomas Deierborg; Laurent Roybon

doi:10.1016/j.stemcr.2015.07.002

Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human Models.

Mehdi Djelloul, Staffan Holmqvist, Antonio Boza Serrano, Carla Azevedo, Maggie S Yeung, Stefano Goldwurm, Jonas Frisén, Tomas Deierborg, Laurent Roybon

Karolinska Institute

Research output: Contribution to journal › Article › peer-review

379 Downloads (Pure)

Abstract

In this study, we sought evidence for alpha-synuclein (ASYN) expression in oligodendrocytes, as a possible endogenous source of ASYN to explain its presence in glial inclusions found in multiple system atrophy (MSA) and Parkinson's disease (PD). We identified ASYN in oligodendrocyte lineage progenitors isolated from the rodent brain, in oligodendrocytes generated from embryonic stem cells, and in induced pluripotent stem cells produced from fibroblasts of a healthy individual and patients diagnosed with MSA or PD, in cultures in vitro. Notably, we observed a significant decrease in ΑSYN during oligodendrocyte maturation. Additionally, we show the presence of transcripts in PDGFRΑ/CD140a(+) cells and SOX10(+) oligodendrocyte lineage nuclei isolated by FACS from rodent and human healthy and diseased brains, respectively. Our work identifies ASYN in oligodendrocyte lineage cells, and it offers additional in vitro cellular models that should provide significant insights of the functional implication of ASYN during oligodendrocyte development and disease.

Original language	English
Pages (from-to)	174-184
Journal	Stem Cell Reports
Volume	5
Issue number	2
DOIs	https://doi.org/10.1016/j.stemcr.2015.07.002
Publication status	Published - 2015

Subject classification (UKÄ)

Cell and Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.stemcr.2015.07.002

Cell_press_Djelloul

http://www.ncbi.nlm.nih.gov/pubmed/26235891?dopt=Abstract

Cite this

@article{f84974afac7e42e3b6ba751c123afe19,

title = "Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human Models.",

abstract = "In this study, we sought evidence for alpha-synuclein (ASYN) expression in oligodendrocytes, as a possible endogenous source of ASYN to explain its presence in glial inclusions found in multiple system atrophy (MSA) and Parkinson's disease (PD). We identified ASYN in oligodendrocyte lineage progenitors isolated from the rodent brain, in oligodendrocytes generated from embryonic stem cells, and in induced pluripotent stem cells produced from fibroblasts of a healthy individual and patients diagnosed with MSA or PD, in cultures in vitro. Notably, we observed a significant decrease in ΑSYN during oligodendrocyte maturation. Additionally, we show the presence of transcripts in PDGFRΑ/CD140a(+) cells and SOX10(+) oligodendrocyte lineage nuclei isolated by FACS from rodent and human healthy and diseased brains, respectively. Our work identifies ASYN in oligodendrocyte lineage cells, and it offers additional in vitro cellular models that should provide significant insights of the functional implication of ASYN during oligodendrocyte development and disease.",

author = "Mehdi Djelloul and Staffan Holmqvist and \{Boza Serrano\}, Antonio and Carla Azevedo and Yeung, \{Maggie S\} and Stefano Goldwurm and Jonas Fris{\'e}n and Tomas Deierborg and Laurent Roybon",

year = "2015",

doi = "10.1016/j.stemcr.2015.07.002",

language = "English",

volume = "5",

pages = "174--184",

journal = "Stem Cell Reports",

issn = "2213-6711",

publisher = "Cell Press",

number = "2",

}

TY - JOUR

T1 - Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human Models.

AU - Djelloul, Mehdi

AU - Holmqvist, Staffan

AU - Boza Serrano, Antonio

AU - Azevedo, Carla

AU - Yeung, Maggie S

AU - Goldwurm, Stefano

AU - Frisén, Jonas

AU - Deierborg, Tomas

AU - Roybon, Laurent

PY - 2015

Y1 - 2015

N2 - In this study, we sought evidence for alpha-synuclein (ASYN) expression in oligodendrocytes, as a possible endogenous source of ASYN to explain its presence in glial inclusions found in multiple system atrophy (MSA) and Parkinson's disease (PD). We identified ASYN in oligodendrocyte lineage progenitors isolated from the rodent brain, in oligodendrocytes generated from embryonic stem cells, and in induced pluripotent stem cells produced from fibroblasts of a healthy individual and patients diagnosed with MSA or PD, in cultures in vitro. Notably, we observed a significant decrease in ΑSYN during oligodendrocyte maturation. Additionally, we show the presence of transcripts in PDGFRΑ/CD140a(+) cells and SOX10(+) oligodendrocyte lineage nuclei isolated by FACS from rodent and human healthy and diseased brains, respectively. Our work identifies ASYN in oligodendrocyte lineage cells, and it offers additional in vitro cellular models that should provide significant insights of the functional implication of ASYN during oligodendrocyte development and disease.

AB - In this study, we sought evidence for alpha-synuclein (ASYN) expression in oligodendrocytes, as a possible endogenous source of ASYN to explain its presence in glial inclusions found in multiple system atrophy (MSA) and Parkinson's disease (PD). We identified ASYN in oligodendrocyte lineage progenitors isolated from the rodent brain, in oligodendrocytes generated from embryonic stem cells, and in induced pluripotent stem cells produced from fibroblasts of a healthy individual and patients diagnosed with MSA or PD, in cultures in vitro. Notably, we observed a significant decrease in ΑSYN during oligodendrocyte maturation. Additionally, we show the presence of transcripts in PDGFRΑ/CD140a(+) cells and SOX10(+) oligodendrocyte lineage nuclei isolated by FACS from rodent and human healthy and diseased brains, respectively. Our work identifies ASYN in oligodendrocyte lineage cells, and it offers additional in vitro cellular models that should provide significant insights of the functional implication of ASYN during oligodendrocyte development and disease.

UR - https://www.scopus.com/pages/publications/84954168300

U2 - 10.1016/j.stemcr.2015.07.002

DO - 10.1016/j.stemcr.2015.07.002

M3 - Article

C2 - 26235891

SN - 2213-6711

VL - 5

SP - 174

EP - 184

JO - Stem Cell Reports

JF - Stem Cell Reports

IS - 2

ER -

Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human Models.

Abstract

Subject classification (UKÄ)

UN SDGs

Access to Document

Other files and links

Fingerprint

Oligodendroglial (dys)function in alpha-synucleinopathies

Generating new models to study propagation and pathology of alpha-synuclein in Parkinson's disease and multiple system atrophy

Cite this