alpha(1)-Antitrypsin Inhibits Epithelial Na+ Transport In Vitro and In Vivo

Ahmed Lazrak, Izabella Nita, Devipriya Subramaniyam, Shipeng Wei, Weifeng Song, Hong-Long Ji, Sabina Janciauskiene, Sadis Matalon

Research output: Contribution to journalArticlepeer-review

30 Citations (SciVal)

Abstract

A variety of studies have shown that Na+ reabsorption across epithelial cells depends on the protease-antiprotease balance. Herein, we investigate the mechanisms by which alpha(1)-antitrypsin (A1AT), a major anti-serine protease in human plasma and lung epithelial fluid and lacking a Kunitz domain, regulates amiloride-sensitive epithelial Na+ channel (ENaC) function in vitro and in vivo. A1AT (0.05 mg/ml = 1 mu M) decreased ENaC currents across Xenopus laevis oocytes injected with human alpha, beta, gamma-ENaC (hENaC) cRNAs, and human lung Clara-like (H441) cells expressing native ENaC, in a partially irreversible fashion. MAT also decreased ENaC single-channel activity when added in the pipette but not in the bath solutions of ENaC-expressing oocytes patched in the cell-attached mode. Incubation of A1AT with peroxynitrite (ONOO-), an oxidizing and nitrating agent, abolished its antiprotease activity and significantly decreased its ability to inhibit ENaC. Intratracheal instillation of normal but not ONOO--treated A1AT (1 mu M) in C57BL/6 mice also decreased Na+-dependent alveolar fluid clearance to the same level as amiloride. Incubation of either H441 cells or ENaC-expressing oocytes with normal but not ONOO--treated MAT decreased their ability to cleave a substrate of serine proteases. A1AT had no effect on amiloride-sensitive currents of oocytes injected with hENaC bearing Liddle mutations, presumably because these channels remain at the surface longer than the wild-type channels. These data indicate that MAT may be an important modulator of ENaC activity and of Na+-dependent fluid clearance across the distal lung epithelium in vivo by decreasing endogenous protease activity needed to activate silent ENaC.
Original languageEnglish
Pages (from-to)261-270
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume41
Issue number3
DOIs
Publication statusPublished - 2009

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Emergency medicine/Medicine/Surgery (013240200), Chronic Inflammatory and Degenerative Diseases Research Unit (013242530)

Subject classification (UKÄ)

  • Cell and Molecular Biology

Keywords

  • Xenopus
  • H441 cells
  • alveolar fluid clearance
  • serine proteases
  • oocytes
  • ENaC

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