Abstract
A new amperometric biosensor for ethanol, based on carbon paste electrode modified with alcohol dehydrogenase (ADH), polyethylenimine (PEI) and using a phenothiazine derivative (DDDP; 16H,18H-dibenzo[c,1]7,9-dithia-16,18-diazapentacene) as redox mediator for NADH recycling, was developed. The biosensor response is the result of mediated oxidation of NADH, generated in the enzymatic reaction between ADH and ethanol (in the presence of NAD(+)). The biosensor sensitivity (calculated as the ratio I-max/K-M(app)) was 0.035 mA M-1 and the detection limit was 0.26 mM, while the linear response range was from 0.1 to 20 mM ethanol.
| Original language | English |
|---|---|
| Pages (from-to) | 55-62 |
| Journal | Studia Universitatis Babes-Bolyai, Chemia |
| Volume | 53 |
| Issue number | 1 |
| Publication status | Published - 2008 |
Bibliographical note
The information about affiliations in this record was updated in December 2015.The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
Subject classification (UKÄ)
- Analytical Chemistry
Free keywords
- phenothiazine derivative
- adenine dinucleotide
- nicotinamide
- ethanol
- amperometric biosensors
- alcohol dehydrogenase
- polyethyleneimine
- modified carbon paste electrodes