An AC Electrokinetic Device for the rapid separation and detection of cancer related DNA nanoparticulate biomarkers

Rajaram Krishnan; David Charlot; Lucas Kumosa; William Hanna; Jerry Lu; Avery Sonnenberg; Michael Heller

doi:10.1109/BioCAS.2011.6107805

An AC Electrokinetic Device for the rapid separation and detection of cancer related DNA nanoparticulate biomarkers

Rajaram Krishnan, David Charlot, Lucas Kumosa, William Hanna, Jerry Lu, Avery Sonnenberg, Michael Heller

Research output: Chapter in Book/Report/Conference proceeding › Paper in conference proceeding › peer-review

Abstract

The ability to rapidly detect cell free circulating (cfc) DNA and other nanoparticulate disease biomarkers directly in blood is a major challenge for nanomedicine. We now show that AC Electrokinetic (ACE) microelectrode array devices can be used to rapidly isolate and detect cfc-DNA nanoparticulates directly from whole blood and other high conductance samples (plasma, serum etc.). The device shows isolation of naked DNA in water and plasma at >500bp size and can isolate DNA down to 50 picograms in 50uL of fluid. Disease specific cfc-DNA materials could also be detected directly in blood from patients with Chronic Lymphocytic Leukemia.

Original language	English
Title of host publication	Biomedical Circuits and Systems Conference (BioCAS), 2011 IEEE
Place of Publication	San Diego
Pages	373-376
DOIs	https://doi.org/10.1109/BioCAS.2011.6107805
Publication status	Published - 2011
Externally published	Yes
Event	2011 IEEE Biomedical Circuits and Systems Conference, BioCAS 2011 - San Diego, CA, United States Duration: 2011 Nov 10 → 2011 Nov 12

Conference

Conference	2011 IEEE Biomedical Circuits and Systems Conference, BioCAS 2011
Country/Territory	United States
City	San Diego, CA
Period	2011/11/10 → 2011/11/12

Subject classification (UKÄ)

Biomedical Laboratory Science/Technology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1109/BioCAS.2011.6107805

Cite this

@inproceedings{b33c3e7e65bc4b5e9c81f229b44837ea,

title = "An AC Electrokinetic Device for the rapid separation and detection of cancer related DNA nanoparticulate biomarkers",

abstract = "The ability to rapidly detect cell free circulating (cfc) DNA and other nanoparticulate disease biomarkers directly in blood is a major challenge for nanomedicine. We now show that AC Electrokinetic (ACE) microelectrode array devices can be used to rapidly isolate and detect cfc-DNA nanoparticulates directly from whole blood and other high conductance samples (plasma, serum etc.). The device shows isolation of naked DNA in water and plasma at >500bp size and can isolate DNA down to 50 picograms in 50uL of fluid. Disease specific cfc-DNA materials could also be detected directly in blood from patients with Chronic Lymphocytic Leukemia.",

author = "Rajaram Krishnan and David Charlot and Lucas Kumosa and William Hanna and Jerry Lu and Avery Sonnenberg and Michael Heller",

year = "2011",

doi = "10.1109/BioCAS.2011.6107805",

language = "English",

isbn = "9781457714696",

pages = "373--376",

booktitle = "Biomedical Circuits and Systems Conference (BioCAS), 2011 IEEE",

note = "2011 IEEE Biomedical Circuits and Systems Conference, BioCAS 2011 ; Conference date: 10-11-2011 Through 12-11-2011",

}

Krishnan, R, Charlot, D, Kumosa, L, Hanna, W, Lu, J, Sonnenberg, A & Heller, M 2011, An AC Electrokinetic Device for the rapid separation and detection of cancer related DNA nanoparticulate biomarkers. in Biomedical Circuits and Systems Conference (BioCAS), 2011 IEEE., 6107805, San Diego, pp. 373-376, 2011 IEEE Biomedical Circuits and Systems Conference, BioCAS 2011, San Diego, CA, United States, 2011/11/10. https://doi.org/10.1109/BioCAS.2011.6107805

An AC Electrokinetic Device for the rapid separation and detection of cancer related DNA nanoparticulate biomarkers. / Krishnan, Rajaram; Charlot, David; Kumosa, Lucas et al.
Biomedical Circuits and Systems Conference (BioCAS), 2011 IEEE. San Diego, 2011. p. 373-376 6107805.

Research output: Chapter in Book/Report/Conference proceeding › Paper in conference proceeding › peer-review

TY - GEN

T1 - An AC Electrokinetic Device for the rapid separation and detection of cancer related DNA nanoparticulate biomarkers

AU - Krishnan, Rajaram

AU - Charlot, David

AU - Kumosa, Lucas

AU - Hanna, William

AU - Lu, Jerry

AU - Sonnenberg, Avery

AU - Heller, Michael

PY - 2011

Y1 - 2011

N2 - The ability to rapidly detect cell free circulating (cfc) DNA and other nanoparticulate disease biomarkers directly in blood is a major challenge for nanomedicine. We now show that AC Electrokinetic (ACE) microelectrode array devices can be used to rapidly isolate and detect cfc-DNA nanoparticulates directly from whole blood and other high conductance samples (plasma, serum etc.). The device shows isolation of naked DNA in water and plasma at >500bp size and can isolate DNA down to 50 picograms in 50uL of fluid. Disease specific cfc-DNA materials could also be detected directly in blood from patients with Chronic Lymphocytic Leukemia.

AB - The ability to rapidly detect cell free circulating (cfc) DNA and other nanoparticulate disease biomarkers directly in blood is a major challenge for nanomedicine. We now show that AC Electrokinetic (ACE) microelectrode array devices can be used to rapidly isolate and detect cfc-DNA nanoparticulates directly from whole blood and other high conductance samples (plasma, serum etc.). The device shows isolation of naked DNA in water and plasma at >500bp size and can isolate DNA down to 50 picograms in 50uL of fluid. Disease specific cfc-DNA materials could also be detected directly in blood from patients with Chronic Lymphocytic Leukemia.

UR - http://www.scopus.com/inward/record.url?scp=84862951501&partnerID=8YFLogxK

U2 - 10.1109/BioCAS.2011.6107805

DO - 10.1109/BioCAS.2011.6107805

M3 - Paper in conference proceeding

AN - SCOPUS:84862951501

SN - 9781457714696

SP - 373

EP - 376

BT - Biomedical Circuits and Systems Conference (BioCAS), 2011 IEEE

CY - San Diego

T2 - 2011 IEEE Biomedical Circuits and Systems Conference, BioCAS 2011

Y2 - 10 November 2011 through 12 November 2011

ER -

An AC Electrokinetic Device for the rapid separation and detection of cancer related DNA nanoparticulate biomarkers

Abstract

Conference

Subject classification (UKÄ)

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this