An integrative toolbox to unlock the structure and dynamics of protein-surfactant complexes

Adrian Sanchez-Fernandez, Carl Diehl, Judith E. Houston, Anna E. Leung, James P. Tellam, Sarah E. Rogers, Sylvain Prevost, Stefan Ulvenlund, Helen Sjögren, Marie Wahlgren

Research output: Contribution to journalArticlepeer-review


The interactions between protein and surfactants play an important role in the stability and performance of formulated products. Due to the high complexity of such interactions, multi-technique approaches are required to study these systems. Here, an integrative approach is used to investigate the various interactions in a model system composed of human growth hormone and sodium dodecyl sulfate. Contrast variation small-angle neutron scattering was used to obtain information on the structure of the protein, surfactant aggregates and surfactant-protein complexes. 1H and 1H-13C HSQC nuclear magnetic resonance spectroscopy was employed to probe the local structure and dynamics of specific amino acids upon surfactant addition. Through the combination of these advanced methods with fluorescence spectroscopy, circular dichroism and isothermal titration calorimetry, it was possible to identify the interaction mechanisms between the surfactant and the protein in the pre- and post-micellar regimes, and interconnect the results from different techniques. As such, the protein was revealed to evolve from a partially unfolded conformation at low SDS concentration to a molten globule at intermediate concentrations, where the protein conformation and local dynamics of hydrophobic amino acids are partially affected compared to the native state. At higher surfactant concentrations the local structure of the protein appears disrupted, and a decorated micelle structure is observed, where the protein is wrapped around a surfactant assembly. Importantly, this integrative approach allows for the identification of the characteristic fingerprints of complex transitions as seen by each technique, and establishes a methodology for an in-detail study of surfactant-protein systems. This journal is

Original languageEnglish
Pages (from-to)4011-4023
Number of pages13
JournalNanoscale Advances
Issue number9
Publication statusPublished - 2020

Subject classification (UKÄ)

  • Materials Chemistry
  • Nano Technology


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