Analyses of the complex formation of staphylococcal enterotoxin A and the human gp130 cytokine receptor

Sibel Uzuncayir; Arturo  Vera-Rodriguez; Paulina Regenthal; Hannah Åbacka; Cecilia Emanuelsson; Christopher D. Bahl; Karin Lindkvist-Petersson

doi:10.1002/1873-3468.14292

Analyses of the complex formation of staphylococcal enterotoxin A and the human gp130 cytokine receptor

Sibel Uzuncayir, Arturo Vera-Rodriguez, Paulina Regenthal, Hannah Åbacka, Cecilia Emanuelsson, Christopher D. Bahl, Karin Lindkvist-Petersson

Research output: Contribution to journal › Article › peer-review

Abstract

Superantigens (SAgs) are bacterial enterotoxins produced by Staphylococcus aureus. Staphylococcal enterotoxin type A (SEA), a staphylococcal superantigen, has been shown to bind to the cytokine signalling receptor glycoprotein 130 (gp130). The structural details, as well as the exact physiological role of this interaction, remain unclear. Here, we describe the structural details of the SEA–gp130 complex by combining crosslinking mass spectrometry and computational modelling. Interestingly, SEA is not able to bind gp130-homologues from rat and mouse. Our data suggest that SEA may interact with human gp130 in a different manner than other known gp130-ligands. Moreover, the fact that SEA does not bind mouse or rat gp130 suggests that SAgs have additional mechanisms of action in humans.

Original language	English
Pages (from-to)	910-923
Journal	FEBS Letters
Volume	596
Issue number	7
Early online date	2022
DOIs	https://doi.org/10.1002/1873-3468.14292
Publication status	Published - 2022

Subject classification (UKÄ)

Immunology in the Medical Area (including Cell and Immunotherapy)
Microbiology in the Medical Area

Access to Document

10.1002/1873-3468.14292Licence: CC BY

1 Doctoral Thesis (compilation)

Dangerous Proteins and Where to Find Them- Structural and functional studies of bacterial and viral proteins interacting with human immune receptors in health and disease
Uzuncayir, S., 2022, Lund: Lund University, Faculty of Medicine. 77 p.
Research output: Thesis › Doctoral Thesis (compilation)

Open Access
File

Cite this

@article{426d5761c245452a9de1a8e558af10d2,

title = "Analyses of the complex formation of staphylococcal enterotoxin A and the human gp130 cytokine receptor",

abstract = "Superantigens (SAgs) are bacterial enterotoxins produced by Staphylococcus aureus. Staphylococcal enterotoxin type A (SEA), a staphylococcal superantigen, has been shown to bind to the cytokine signalling receptor glycoprotein 130 (gp130). The structural details, as well as the exact physiological role of this interaction, remain unclear. Here, we describe the structural details of the SEA–gp130 complex by combining crosslinking mass spectrometry and computational modelling. Interestingly, SEA is not able to bind gp130-homologues from rat and mouse. Our data suggest that SEA may interact with human gp130 in a different manner than other known gp130-ligands. Moreover, the fact that SEA does not bind mouse or rat gp130 suggests that SAgs have additional mechanisms of action in humans.",

author = "Sibel Uzuncayir and Arturo Vera-Rodriguez and Paulina Regenthal and Hannah {\AA}backa and Cecilia Emanuelsson and Bahl, {Christopher D.} and Karin Lindkvist-Petersson",

year = "2022",

doi = "10.1002/1873-3468.14292",

language = "English",

volume = "596",

pages = "910--923",

journal = "FEBS Letters",

issn = "1873-3468",

publisher = "Wiley-Blackwell",

number = "7",

}

TY - JOUR

T1 - Analyses of the complex formation of staphylococcal enterotoxin A and the human gp130 cytokine receptor

AU - Uzuncayir, Sibel

AU - Vera-Rodriguez, Arturo

AU - Regenthal, Paulina

AU - Åbacka, Hannah

AU - Emanuelsson, Cecilia

AU - Bahl, Christopher D.

AU - Lindkvist-Petersson, Karin

PY - 2022

Y1 - 2022

N2 - Superantigens (SAgs) are bacterial enterotoxins produced by Staphylococcus aureus. Staphylococcal enterotoxin type A (SEA), a staphylococcal superantigen, has been shown to bind to the cytokine signalling receptor glycoprotein 130 (gp130). The structural details, as well as the exact physiological role of this interaction, remain unclear. Here, we describe the structural details of the SEA–gp130 complex by combining crosslinking mass spectrometry and computational modelling. Interestingly, SEA is not able to bind gp130-homologues from rat and mouse. Our data suggest that SEA may interact with human gp130 in a different manner than other known gp130-ligands. Moreover, the fact that SEA does not bind mouse or rat gp130 suggests that SAgs have additional mechanisms of action in humans.

AB - Superantigens (SAgs) are bacterial enterotoxins produced by Staphylococcus aureus. Staphylococcal enterotoxin type A (SEA), a staphylococcal superantigen, has been shown to bind to the cytokine signalling receptor glycoprotein 130 (gp130). The structural details, as well as the exact physiological role of this interaction, remain unclear. Here, we describe the structural details of the SEA–gp130 complex by combining crosslinking mass spectrometry and computational modelling. Interestingly, SEA is not able to bind gp130-homologues from rat and mouse. Our data suggest that SEA may interact with human gp130 in a different manner than other known gp130-ligands. Moreover, the fact that SEA does not bind mouse or rat gp130 suggests that SAgs have additional mechanisms of action in humans.

U2 - 10.1002/1873-3468.14292

DO - 10.1002/1873-3468.14292

M3 - Article

C2 - 35060124

SN - 1873-3468

VL - 596

SP - 910

EP - 923

JO - FEBS Letters

JF - FEBS Letters

IS - 7

ER -

Analyses of the complex formation of staphylococcal enterotoxin A and the human gp130 cytokine receptor

Abstract

Subject classification (UKÄ)

Access to Document

Fingerprint

Research output

Dangerous Proteins and Where to Find Them- Structural and functional studies of bacterial and viral proteins interacting with human immune receptors in health and disease

Cite this