Analyses of the complex formation of staphylococcal enterotoxin A and the human gp130 cytokine receptor

Sibel Uzuncayir, Arturo Vera-Rodriguez, Paulina Regenthal, Hannah Åbacka, Cecilia Emanuelsson, Christopher D. Bahl, Karin Lindkvist-Petersson

Research output: Contribution to journalArticlepeer-review

Abstract

Superantigens (SAgs) are bacterial enterotoxins produced by Staphylococcus aureus. Staphylococcal enterotoxin type A (SEA), a staphylococcal superantigen, has been shown to bind to the cytokine signalling receptor glycoprotein 130 (gp130). The structural details, as well as the exact physiological role of this interaction, remain unclear. Here, we describe the structural details of the SEA–gp130 complex by combining crosslinking mass spectrometry and computational modelling. Interestingly, SEA is not able to bind gp130-homologues from rat and mouse. Our data suggest that SEA may interact with human gp130 in a different manner than other known gp130-ligands. Moreover, the fact that SEA does not bind mouse or rat gp130 suggests that SAgs have additional mechanisms of action in humans.
Original languageEnglish
Pages (from-to)910-923
JournalFEBS Letters
Volume596
Issue number7
Early online date2022
DOIs
Publication statusPublished - 2022

Subject classification (UKÄ)

  • Immunology in the medical area
  • Microbiology in the medical area

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