Analysis of anti-C1q autoantibodies by western blot

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Abstract

Anti-C1q autoantibodies may be found in many conditions, most commonly in systemic lupus erythematosus (SLE) and hypocomplementemic urticarial vasculitis syndrome (HUVS), and are diagnostic markers as well as disease activity markers in lupus nephritis. Sera from patients with SLE and HUVS show partly distinct autoantibody reactivities to separated protein chains B and C of the first component of complement, C1q. These different binding specificities can be detected by Western blot analysis of the autoantibodies under reducing conditions. Results may help clinicians to differentiate between SLE and HUVS.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages183-189
Number of pages7
DOIs
Publication statusPublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1901
ISSN (Print)1064-3745

Subject classification (UKÄ)

  • Rheumatology and Autoimmunity

Free keywords

  • Anti-C1q autoantibodies
  • C1q protein chains B and C
  • Hypocomplementemic urticarial vasculitis syndrome
  • Systemic lupus erythematosus
  • Western blot

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