Analysis of C3 nephritic factors by ELISA

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

Autoantibodies to the C3 convertase of the alternative pathway of complement, called C3 nephritic factors (C3NeF), cause persistently low C3 in the circulation and production of C3 degradation fragments due to prolonged stabilization of the C3 convertase. C3NeF are associated with glomerulopathy, acquired partial lipodystrophy, and less frequently with increased susceptibility to meningococcal infection. Analysis of C3NeF is an important part of the diagnostic workup of C3 glomerulopathy, but their identification is difficult presumably due to considerable heterogeneity. Therefore it is recommended to use a combination of different analysis methods for the detection of C3NeF. Here we present an ELISA method for detection of C3NeF.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages177-182
Number of pages6
DOIs
Publication statusPublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1901
ISSN (Print)1064-3745

Subject classification (UKÄ)

  • Rheumatology and Autoimmunity

Free keywords

  • Alternative pathway convertase
  • Autoantibodies to complement
  • C3 glomerulopathy
  • C3 nephritic factors
  • ELISA

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