Antibodies to merkel cell polyomavirus correlate to presence of viral DNA in the skin.

Helena Faust; Diana V Pastrana; Christopher B Buck; Joakim Dillner; Johanna Ekström

doi:10.1093/infdis/jiq173

Antibodies to merkel cell polyomavirus correlate to presence of viral DNA in the skin.

Helena Faust, Diana V Pastrana, Christopher B Buck, Joakim Dillner, Johanna Ekström

Clinical Microbiology, Malmö

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Abstract

To validate whether Merkel cell polyomavirus (MCV) serology correlates with MCV infection, we compared real-time polymerase chain reaction results for MCV DNA on fresh-frozen biopsy specimens from various skin lesions and healthy skin from 434 patients to MCV serology results using viruslike particles (VLPs) and MCV neutralization assays. Sixty-five percent of participants were MCV seropositive and 18% were MCV DNA positive. The presence of antibodies was correlated with the presence of virus DNA (odds ratio, 27.85 [95% confidence interval, 6.6-166.5]), with 97% of patients who tested positive for MCV DNA being MCV seropositive. VLP antibody levels correlated to neutralization titers (r = .72), and high antibody levels correlated to high MCV load (P < .01).

Original language	English
Pages (from-to)	1096-1100
Journal	Journal of Infectious Diseases
Volume	203
Issue number	8
DOIs	https://doi.org/10.1093/infdis/jiq173
Publication status	Published - 2011

Subject classification (UKÄ)

Infectious Medicine

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10.1093/infdis/jiq173

Oxford_University_Press__Faust_et_al

http://www.ncbi.nlm.nih.gov/pubmed/21450999?dopt=Abstract

1 Doctoral Thesis (compilation)

Pseudovirions in the Study of Papilloma- and Polyomaviruses
Faust, H., 2012, Laboratory Medicine, Medical Microbiology Malmö. 154 p.
Research output: Thesis › Doctoral Thesis (compilation)

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title = "Antibodies to merkel cell polyomavirus correlate to presence of viral DNA in the skin.",

abstract = "To validate whether Merkel cell polyomavirus (MCV) serology correlates with MCV infection, we compared real-time polymerase chain reaction results for MCV DNA on fresh-frozen biopsy specimens from various skin lesions and healthy skin from 434 patients to MCV serology results using viruslike particles (VLPs) and MCV neutralization assays. Sixty-five percent of participants were MCV seropositive and 18% were MCV DNA positive. The presence of antibodies was correlated with the presence of virus DNA (odds ratio, 27.85 [95% confidence interval, 6.6-166.5]), with 97% of patients who tested positive for MCV DNA being MCV seropositive. VLP antibody levels correlated to neutralization titers (r = .72), and high antibody levels correlated to high MCV load (P < .01).",

author = "Helena Faust and Pastrana, {Diana V} and Buck, {Christopher B} and Joakim Dillner and Johanna Ekstr{\"o}m",

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doi = "10.1093/infdis/jiq173",

language = "English",

volume = "203",

pages = "1096--1100",

journal = "Journal of Infectious Diseases",

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publisher = "Oxford University Press",

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TY - JOUR

T1 - Antibodies to merkel cell polyomavirus correlate to presence of viral DNA in the skin.

AU - Faust, Helena

AU - Pastrana, Diana V

AU - Buck, Christopher B

AU - Dillner, Joakim

AU - Ekström, Johanna

PY - 2011

Y1 - 2011

N2 - To validate whether Merkel cell polyomavirus (MCV) serology correlates with MCV infection, we compared real-time polymerase chain reaction results for MCV DNA on fresh-frozen biopsy specimens from various skin lesions and healthy skin from 434 patients to MCV serology results using viruslike particles (VLPs) and MCV neutralization assays. Sixty-five percent of participants were MCV seropositive and 18% were MCV DNA positive. The presence of antibodies was correlated with the presence of virus DNA (odds ratio, 27.85 [95% confidence interval, 6.6-166.5]), with 97% of patients who tested positive for MCV DNA being MCV seropositive. VLP antibody levels correlated to neutralization titers (r = .72), and high antibody levels correlated to high MCV load (P < .01).

AB - To validate whether Merkel cell polyomavirus (MCV) serology correlates with MCV infection, we compared real-time polymerase chain reaction results for MCV DNA on fresh-frozen biopsy specimens from various skin lesions and healthy skin from 434 patients to MCV serology results using viruslike particles (VLPs) and MCV neutralization assays. Sixty-five percent of participants were MCV seropositive and 18% were MCV DNA positive. The presence of antibodies was correlated with the presence of virus DNA (odds ratio, 27.85 [95% confidence interval, 6.6-166.5]), with 97% of patients who tested positive for MCV DNA being MCV seropositive. VLP antibody levels correlated to neutralization titers (r = .72), and high antibody levels correlated to high MCV load (P < .01).

U2 - 10.1093/infdis/jiq173

DO - 10.1093/infdis/jiq173

M3 - Article

C2 - 21450999

SN - 1537-6613

VL - 203

SP - 1096

EP - 1100

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

IS - 8

ER -

Antibodies to merkel cell polyomavirus correlate to presence of viral DNA in the skin.

Abstract

Subject classification (UKÄ)

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Research output

Pseudovirions in the Study of Papilloma- and Polyomaviruses

Cite this