Research output per year
Research output per year
Alexandra Aidoukovitch, Sara Dahl, Felicia Fält, Daniel Nebel, Daniel Svensson, Ellen Tufvesson, Bengt-Olof Nilsson
Research output: Contribution to journal › Article › peer-review
The antimicrobial peptide LL-37 is active against oral bacteria and has been demonstrated to be present in human saliva, but its distribution in different fractions of saliva is not known. LL-37 is formed from its intracellular pro-form, hCAP18, in an extracellular enzymatic reaction catalyzed by proteinase 3 and kallikrein 5. Here, we prepared cell-containing and cell-free fractions of unstimulated human whole saliva by centrifugation after depolymerization of mucins with dithiothreitol, and measured the levels of hCAP18/LL-37 in these fractions using ELISA. Cellular expression of hCAP18/LL-37 was determined by western blotting and immunocytochemistry. The ELISA analyses demonstrated that both cells and cell-free saliva contained hCAP18/LL-37. Western blot analysis of cell-pellet homogenates showed a strong band corresponding to hCAP18 at the correct molecular weight and a weak band corresponding to LL-37. Phase-contrast and light microscopy revealed that the cells consisted of desquamated epithelial cells. These cells expressed cytoplasmic immunoreactivity for hCAP18/LL-37. The peripheral part of the cytoplasm, corresponding to the plasma membrane, was particularly rich in hCAP18/LL-37 immunoreactivity. No immunoreactivity was observed after omission of the primary antibody. We conclude that desquamated epithelial cells of human whole saliva contain antimicrobial hCAP18/LL-37, suggesting that these cells may take part in the innate immune system by harboring and releasing these peptides.
Original language | English |
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Pages (from-to) | 1-6 |
Number of pages | 6 |
Journal | European Journal of Oral Sciences |
Volume | 128 |
Issue number | 1 |
Early online date | 2019 Dec 11 |
DOIs | |
Publication status | Published - 2020 Feb |
Research output: Thesis › Doctoral Thesis (compilation)
Aidoukovitch, A. (Research student) & Nilsson, B.-O. (Supervisor)
The Royal Physiographic Society in Lund
2016/11/01 → 2023/06/01
Project: Dissertation