Association and Interactions in Protein-Surfactant Systems

Anna Stenstam

Association and Interactions in Protein-Surfactant Systems

Anna Stenstam

Physical Chemistry

Research output: Thesis › Doctoral Thesis (compilation)

Abstract

Aggregation in protein-surfactant systems have been studied with the ambition of reaching a deeper understanding of the balance of the interaction forces involved. A pure system with maximized electrostatic interactions has been used. The approach has been to use a step-wise sample preparation where initially a stoichiometric protein-surfactant salt is precipitated and freeze-dried. Secondly, the solubilization of the protein-surfactant salts has been studied both in excess of the parent surfactant and by addition of another surfactant. The mechanisms for solubilization in the two cases are not the same but dependent on the surfactant and surfactant-surfactant aggregation behavior, respectively. The solubilization is studied by Nuclear Magnetic Resonance (NMR) diffusometry, Raman spectroscopy and UV absorption spectroscopy. Further, the gel formed in the lysozyme-alkylsulfate-water systems is analyzed in terms of both the question of its thermodynamic status and its structure. The latter is approached by Small Angle Neutron Scattering (SANS) and Transmission Electron Microscopy (TEM). Finally, the more complex system of the surfactant aggregation with the photosynthetic light-harvesting antenna (LH2) is studied in the light of the surfactant self-aggregation pattern.

Original language	English
Qualification	Doctor
Awarding Institution	Physical Chemistry
Supervisors/Advisors	[unknown], [unknown], Supervisor, External person
Award date	2003 Oct 24
Publisher	Håkan Wennerström, Physical Chemistry 1, Chemical Centre Lund University
ISBN (Print)	91-628-5801-7
Publication status	Published - 2003

Bibliographical note

Defence details

Date: 2003-10-24
Time: 10:15
Place: Chemical Centre, Lecture hall B

External reviewer(s)

Name: Lindblom, Göran
Title: Professor
Affiliation: Department of Chemistry, Biophysical Chemistry, Umeå University

---

Article: I. The Lysozyme-Dodecyl Sulfate System. An Example of Protein-Surfactant Aggregation.Anna Stenstam, Ali Khan and Håkan Wennerström.Langmuir (2001) 17(24), 7513-7520.

Article: II. Aggregation in a Protein-Surfactant System. The Interplay Between Hydrophobic and Electrostatic Interactions.Anna Stenstam, Daniel Topgaard and Håkan Wennerström.J. Phys. Chem. B. (2003), 107, 7987-7992.

Article: III. A SANS Study of Lysozyme-SDS Aggregates.Anna Stenstam, Gemma Montalvo, Isabelle Grillo and Michael Gradzielski.Accepted for publication in J. Phys. Chem. B.

Article: IV. The Triangle Drama of Protein in Catanionic Surfactant Mixtures.Anna Stenstam, Ali Khan and Håkan WennerströmSubmitted to Langmuir.

Article: V. Self-assembly of the Bacterial Photosynthetic Pigment-Protein LH2 Revealed by Ultrafast Spectroscopy.Axel Schubert, Anna Stenstam, Wichard J. D. Beenken, Tõnu Pullerits, Jennifer L. Herek, Richard Cogdell and Villy Sundström.To be submitted to Biophysical Journal.

Subject classification (UKÄ)

Physical Chemistry (including Surface- and Colloid Chemistry)

Free keywords

catanionic surfactant
SANS
NMR
gel
sodium alkylsulfate
Protein-surfactant interactions
lysozyme
LH2
Physical chemistry
Fysikalisk kemi

Cite this

@phdthesis{21fb4981a7a34eb0a71c05d0098ace94,

title = "Association and Interactions in Protein-Surfactant Systems",

abstract = "Aggregation in protein-surfactant systems have been studied with the ambition of reaching a deeper understanding of the balance of the interaction forces involved. A pure system with maximized electrostatic interactions has been used. The approach has been to use a step-wise sample preparation where initially a stoichiometric protein-surfactant salt is precipitated and freeze-dried. Secondly, the solubilization of the protein-surfactant salts has been studied both in excess of the parent surfactant and by addition of another surfactant. The mechanisms for solubilization in the two cases are not the same but dependent on the surfactant and surfactant-surfactant aggregation behavior, respectively. The solubilization is studied by Nuclear Magnetic Resonance (NMR) diffusometry, Raman spectroscopy and UV absorption spectroscopy. Further, the gel formed in the lysozyme-alkylsulfate-water systems is analyzed in terms of both the question of its thermodynamic status and its structure. The latter is approached by Small Angle Neutron Scattering (SANS) and Transmission Electron Microscopy (TEM). Finally, the more complex system of the surfactant aggregation with the photosynthetic light-harvesting antenna (LH2) is studied in the light of the surfactant self-aggregation pattern.",

keywords = "catanionic surfactant, SANS, NMR, gel, sodium alkylsulfate, Protein-surfactant interactions, lysozyme, LH2, Physical chemistry, Fysikalisk kemi",

author = "Anna Stenstam",

note = "Defence details Date: 2003-10-24 Time: 10:15 Place: Chemical Centre, Lecture hall B External reviewer(s) Name: Lindblom, G{\"o}ran Title: Professor Affiliation: Department of Chemistry, Biophysical Chemistry, Ume{\aa} University --- Article: I. The Lysozyme-Dodecyl Sulfate System. An Example of Protein-Surfactant Aggregation.Anna Stenstam, Ali Khan and H{\aa}kan Wennerstr{\"o}m.Langmuir (2001) 17(24), 7513-7520. Article: II. Aggregation in a Protein-Surfactant System. The Interplay Between Hydrophobic and Electrostatic Interactions.Anna Stenstam, Daniel Topgaard and H{\aa}kan Wennerstr{\"o}m.J. Phys. Chem. B. (2003), 107, 7987-7992. Article: III. A SANS Study of Lysozyme-SDS Aggregates.Anna Stenstam, Gemma Montalvo, Isabelle Grillo and Michael Gradzielski.Accepted for publication in J. Phys. Chem. B. Article: IV. The Triangle Drama of Protein in Catanionic Surfactant Mixtures.Anna Stenstam, Ali Khan and H{\aa}kan Wennerstr{\"o}mSubmitted to Langmuir. Article: V. Self-assembly of the Bacterial Photosynthetic Pigment-Protein LH2 Revealed by Ultrafast Spectroscopy.Axel Schubert, Anna Stenstam, Wichard J. D. Beenken, T{\~o}nu Pullerits, Jennifer L. Herek, Richard Cogdell and Villy Sundstr{\"o}m.To be submitted to Biophysical Journal.",

year = "2003",

language = "English",

isbn = "91-628-5801-7",

publisher = "H{\aa}kan Wennerstr{\"o}m, Physical Chemistry 1, Chemical Centre Lund University",

type = "Doctoral Thesis (compilation)",

school = "Physical Chemistry",

}

TY - THES

T1 - Association and Interactions in Protein-Surfactant Systems

AU - Stenstam, Anna

N1 - Defence details Date: 2003-10-24 Time: 10:15 Place: Chemical Centre, Lecture hall B External reviewer(s) Name: Lindblom, Göran Title: Professor Affiliation: Department of Chemistry, Biophysical Chemistry, Umeå University --- Article: I. The Lysozyme-Dodecyl Sulfate System. An Example of Protein-Surfactant Aggregation.Anna Stenstam, Ali Khan and Håkan Wennerström.Langmuir (2001) 17(24), 7513-7520. Article: II. Aggregation in a Protein-Surfactant System. The Interplay Between Hydrophobic and Electrostatic Interactions.Anna Stenstam, Daniel Topgaard and Håkan Wennerström.J. Phys. Chem. B. (2003), 107, 7987-7992. Article: III. A SANS Study of Lysozyme-SDS Aggregates.Anna Stenstam, Gemma Montalvo, Isabelle Grillo and Michael Gradzielski.Accepted for publication in J. Phys. Chem. B. Article: IV. The Triangle Drama of Protein in Catanionic Surfactant Mixtures.Anna Stenstam, Ali Khan and Håkan WennerströmSubmitted to Langmuir. Article: V. Self-assembly of the Bacterial Photosynthetic Pigment-Protein LH2 Revealed by Ultrafast Spectroscopy.Axel Schubert, Anna Stenstam, Wichard J. D. Beenken, Tõnu Pullerits, Jennifer L. Herek, Richard Cogdell and Villy Sundström.To be submitted to Biophysical Journal.

PY - 2003

Y1 - 2003

N2 - Aggregation in protein-surfactant systems have been studied with the ambition of reaching a deeper understanding of the balance of the interaction forces involved. A pure system with maximized electrostatic interactions has been used. The approach has been to use a step-wise sample preparation where initially a stoichiometric protein-surfactant salt is precipitated and freeze-dried. Secondly, the solubilization of the protein-surfactant salts has been studied both in excess of the parent surfactant and by addition of another surfactant. The mechanisms for solubilization in the two cases are not the same but dependent on the surfactant and surfactant-surfactant aggregation behavior, respectively. The solubilization is studied by Nuclear Magnetic Resonance (NMR) diffusometry, Raman spectroscopy and UV absorption spectroscopy. Further, the gel formed in the lysozyme-alkylsulfate-water systems is analyzed in terms of both the question of its thermodynamic status and its structure. The latter is approached by Small Angle Neutron Scattering (SANS) and Transmission Electron Microscopy (TEM). Finally, the more complex system of the surfactant aggregation with the photosynthetic light-harvesting antenna (LH2) is studied in the light of the surfactant self-aggregation pattern.

AB - Aggregation in protein-surfactant systems have been studied with the ambition of reaching a deeper understanding of the balance of the interaction forces involved. A pure system with maximized electrostatic interactions has been used. The approach has been to use a step-wise sample preparation where initially a stoichiometric protein-surfactant salt is precipitated and freeze-dried. Secondly, the solubilization of the protein-surfactant salts has been studied both in excess of the parent surfactant and by addition of another surfactant. The mechanisms for solubilization in the two cases are not the same but dependent on the surfactant and surfactant-surfactant aggregation behavior, respectively. The solubilization is studied by Nuclear Magnetic Resonance (NMR) diffusometry, Raman spectroscopy and UV absorption spectroscopy. Further, the gel formed in the lysozyme-alkylsulfate-water systems is analyzed in terms of both the question of its thermodynamic status and its structure. The latter is approached by Small Angle Neutron Scattering (SANS) and Transmission Electron Microscopy (TEM). Finally, the more complex system of the surfactant aggregation with the photosynthetic light-harvesting antenna (LH2) is studied in the light of the surfactant self-aggregation pattern.

KW - catanionic surfactant

KW - SANS

KW - NMR

KW - gel

KW - sodium alkylsulfate

KW - Protein-surfactant interactions

KW - lysozyme

KW - LH2

KW - Physical chemistry

KW - Fysikalisk kemi

M3 - Doctoral Thesis (compilation)

SN - 91-628-5801-7

PB - Håkan Wennerström, Physical Chemistry 1, Chemical Centre Lund University

ER -

Association and Interactions in Protein-Surfactant Systems

Abstract

Bibliographical note

Subject classification (UKÄ)

Free keywords

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Cite this