Abstract
The RBTN2 LIM-domain protein, originally identified as an oncogenic protein in human T-cell leukemia, is essential for erythropoiesis. A possible role for RBTN2 in transcription during erythropoiesis has been investigated. Direct interaction of the RBTN2 protein was observed in vivo and in vitro with the GATA1 or -2 zinc-finger transcription factors, as well as with the basic helix-loop-helix protein TAL1. By using mammalian two-hybrid analysis, complexes involving RBTN2, TAL1, and GATA1, together with E47, the basic helix-loop-helix heterodimerization partner of TAL1, could be demonstrated. Thus, a molecular link exists between three proteins crucial for erythropoiesis, and the data suggest that variations in amounts of complexes involving RBTN2, TAL1, and GATA1 could be important for erythroid differentiation.
| Original language | English |
|---|---|
| Pages (from-to) | 9585-9 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences |
| Volume | 92 |
| Issue number | 21 |
| Publication status | Published - 1995 Oct 10 |
Subject classification (UKÄ)
- Hematology
Free keywords
- Adaptor Proteins, Signal Transducing
- Amino Acid Sequence
- Animals
- Basic Helix-Loop-Helix Transcription Factors
- DNA-Binding Proteins
- Erythroid-Specific DNA-Binding Factors
- Erythropoiesis
- GATA1 Transcription Factor
- Helix-Loop-Helix Motifs
- Homeodomain Proteins
- LIM Domain Proteins
- Leukemia, Erythroblastic, Acute
- Metalloproteins
- Mice
- Molecular Sequence Data
- Protein Binding
- Proto-Oncogene Proteins
- Recombinant Proteins
- TCF Transcription Factors
- Transcription Factor 7-Like 1 Protein
- Transcription Factors
- Tumor Cells, Cultured
- Zinc Fingers