Association of STAT4 with rheumatoid arthritis - A replication study in three European populations

Gisela Orozco; Behrooz Z. Alizadeh; Angelica M. Delgado-Vega; Miguel A. Gonzalez-Gay; Alejandro Balsa; Dora Pascual-Salcedo; Benjamin Fernandez-Gutierrez; Maria F. Gonzalez-Escribano; Ingemar Petersson; Piet L. C. M. van Riel; Pilar Barrera; Marieke J. H. Coenen; Timothy R. D. J. Radstake; Miek A. van Leeuwen; Cisca Wijmenga; Bobby P. C. Koeleman; Marta Alarcon-Riquelme; Javier Martin

doi:10.1002/art.23549

Association of STAT4 with rheumatoid arthritis - A replication study in three European populations

Gisela Orozco, Behrooz Z. Alizadeh, Angelica M. Delgado-Vega, Miguel A. Gonzalez-Gay, Alejandro Balsa, Dora Pascual-Salcedo, Benjamin Fernandez-Gutierrez, Maria F. Gonzalez-Escribano, Ingemar Petersson, Piet L. C. M. van Riel, Pilar Barrera, Marieke J. H. Coenen, Timothy R. D. J. Radstake, Miek A. van Leeuwen, Cisca Wijmenga, Bobby P. C. Koeleman, Marta Alarcon-Riquelme, Javier Martin

Orthopaedics (Lund)

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Abstract

Objective. This study was undertaken to investigate the previously reported association of the STAT4 polymorphism rs7574865 with rheumatoid arthritis (RA) in 3 different European populations from Spain, Sweden, and The Netherlands, comprising a total of 2,072 patients and 2,474 controls. Methods. Three different cohorts were included in the study: 923 RA patients and 1,296 healthy controls from Spain, 273 RA patients and 285 healthy controls from. Sweden, and 876 RA patients and 893 healthy controls from The Netherlands. DNA from patients and controls was obtained from peripheral blood. Samples were genotyped for the STAT4 single-nucleotide polymorphism rs7574865 using a TaqMan 5'-allele discrimination assay. The chi-square test was performed to compare allele and genotype distributions. Odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated. Results. We observed a significantly increased frequency of the minor T allele in RA patients compared with healthy controls in the Spanish population (24.8% versus 20.8%; P = 0.001, OR 1.26 [95% CI 1.09-1.45]). This association was confirmed in both the Swedish population (P = 0.03, OR 1.35 [95% CI 1.03-1.77]) and the Dutch population (P = 0.03, OR 1.45 [95% CI 1.21-1.73]). The overall P value for all 3 populations was 9.79 x 10(-6) (OR 1.25 [95% CI 1.13-1.37]). No association between rs7574865 and the presence of rheumatoid factor or anti-cyclic citrullinated peptide autoantibodies was observed. A meta-analysis of all published STAT4 associations revealed an OR of 1.25 (95% CI 1.19-1.33) (P = 1 x 10(-5)). Conclusion. Our findings indicate an association between the STAT4 polymorphism rs7574865 and RA in 3 different populations, from Spain, Sweden, and The Netherlands, thereby confirming previous data.

Original language	English
Pages (from-to)	1974-1980
Journal	Arthritis and Rheumatism
Volume	58
Issue number	7
DOIs	https://doi.org/10.1002/art.23549
Publication status	Published - 2008

Subject classification (UKÄ)

Rheumatology and Autoimmunity

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10.1002/art.23549

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Orozco, G., Alizadeh, B. Z., Delgado-Vega, A. M., Gonzalez-Gay, M. A., Balsa, A., Pascual-Salcedo, D., Fernandez-Gutierrez, B., Gonzalez-Escribano, M. F., Petersson, I., van Riel, P. L. C. M., Barrera, P., Coenen, M. J. H., Radstake, T. R. D. J., van Leeuwen, M. A., Wijmenga, C., Koeleman, B. P. C., Alarcon-Riquelme, M., & Martin, J. (2008). Association of STAT4 with rheumatoid arthritis - A replication study in three European populations. Arthritis and Rheumatism, 58(7), 1974-1980. https://doi.org/10.1002/art.23549

@article{2dd5301718624aff942b03649bba415b,

title = "Association of STAT4 with rheumatoid arthritis - A replication study in three European populations",

abstract = "Objective. This study was undertaken to investigate the previously reported association of the STAT4 polymorphism rs7574865 with rheumatoid arthritis (RA) in 3 different European populations from Spain, Sweden, and The Netherlands, comprising a total of 2,072 patients and 2,474 controls. Methods. Three different cohorts were included in the study: 923 RA patients and 1,296 healthy controls from Spain, 273 RA patients and 285 healthy controls from. Sweden, and 876 RA patients and 893 healthy controls from The Netherlands. DNA from patients and controls was obtained from peripheral blood. Samples were genotyped for the STAT4 single-nucleotide polymorphism rs7574865 using a TaqMan 5'-allele discrimination assay. The chi-square test was performed to compare allele and genotype distributions. Odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated. Results. We observed a significantly increased frequency of the minor T allele in RA patients compared with healthy controls in the Spanish population (24.8% versus 20.8%; P = 0.001, OR 1.26 [95% CI 1.09-1.45]). This association was confirmed in both the Swedish population (P = 0.03, OR 1.35 [95% CI 1.03-1.77]) and the Dutch population (P = 0.03, OR 1.45 [95% CI 1.21-1.73]). The overall P value for all 3 populations was 9.79 x 10(-6) (OR 1.25 [95% CI 1.13-1.37]). No association between rs7574865 and the presence of rheumatoid factor or anti-cyclic citrullinated peptide autoantibodies was observed. A meta-analysis of all published STAT4 associations revealed an OR of 1.25 (95% CI 1.19-1.33) (P = 1 x 10(-5)). Conclusion. Our findings indicate an association between the STAT4 polymorphism rs7574865 and RA in 3 different populations, from Spain, Sweden, and The Netherlands, thereby confirming previous data.",

author = "Gisela Orozco and Alizadeh, {Behrooz Z.} and Delgado-Vega, {Angelica M.} and Gonzalez-Gay, {Miguel A.} and Alejandro Balsa and Dora Pascual-Salcedo and Benjamin Fernandez-Gutierrez and Gonzalez-Escribano, {Maria F.} and Ingemar Petersson and {van Riel}, {Piet L. C. M.} and Pilar Barrera and Coenen, {Marieke J. H.} and Radstake, {Timothy R. D. J.} and {van Leeuwen}, {Miek A.} and Cisca Wijmenga and Koeleman, {Bobby P. C.} and Marta Alarcon-Riquelme and Javier Martin",

year = "2008",

doi = "10.1002/art.23549",

language = "English",

volume = "58",

pages = "1974--1980",

journal = "Arthritis and Rheumatism",

issn = "1529-0131",

publisher = "John Wiley & Sons Inc.",

number = "7",

}

Orozco, G, Alizadeh, BZ, Delgado-Vega, AM, Gonzalez-Gay, MA, Balsa, A, Pascual-Salcedo, D, Fernandez-Gutierrez, B, Gonzalez-Escribano, MF, Petersson, I, van Riel, PLCM, Barrera, P, Coenen, MJH, Radstake, TRDJ, van Leeuwen, MA, Wijmenga, C, Koeleman, BPC, Alarcon-Riquelme, M & Martin, J 2008, 'Association of STAT4 with rheumatoid arthritis - A replication study in three European populations', Arthritis and Rheumatism, vol. 58, no. 7, pp. 1974-1980. https://doi.org/10.1002/art.23549

TY - JOUR

T1 - Association of STAT4 with rheumatoid arthritis - A replication study in three European populations

AU - Orozco, Gisela

AU - Alizadeh, Behrooz Z.

AU - Delgado-Vega, Angelica M.

AU - Gonzalez-Gay, Miguel A.

AU - Balsa, Alejandro

AU - Pascual-Salcedo, Dora

AU - Fernandez-Gutierrez, Benjamin

AU - Gonzalez-Escribano, Maria F.

AU - Petersson, Ingemar

AU - van Riel, Piet L. C. M.

AU - Barrera, Pilar

AU - Coenen, Marieke J. H.

AU - Radstake, Timothy R. D. J.

AU - van Leeuwen, Miek A.

AU - Wijmenga, Cisca

AU - Koeleman, Bobby P. C.

AU - Alarcon-Riquelme, Marta

AU - Martin, Javier

PY - 2008

Y1 - 2008

N2 - Objective. This study was undertaken to investigate the previously reported association of the STAT4 polymorphism rs7574865 with rheumatoid arthritis (RA) in 3 different European populations from Spain, Sweden, and The Netherlands, comprising a total of 2,072 patients and 2,474 controls. Methods. Three different cohorts were included in the study: 923 RA patients and 1,296 healthy controls from Spain, 273 RA patients and 285 healthy controls from. Sweden, and 876 RA patients and 893 healthy controls from The Netherlands. DNA from patients and controls was obtained from peripheral blood. Samples were genotyped for the STAT4 single-nucleotide polymorphism rs7574865 using a TaqMan 5'-allele discrimination assay. The chi-square test was performed to compare allele and genotype distributions. Odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated. Results. We observed a significantly increased frequency of the minor T allele in RA patients compared with healthy controls in the Spanish population (24.8% versus 20.8%; P = 0.001, OR 1.26 [95% CI 1.09-1.45]). This association was confirmed in both the Swedish population (P = 0.03, OR 1.35 [95% CI 1.03-1.77]) and the Dutch population (P = 0.03, OR 1.45 [95% CI 1.21-1.73]). The overall P value for all 3 populations was 9.79 x 10(-6) (OR 1.25 [95% CI 1.13-1.37]). No association between rs7574865 and the presence of rheumatoid factor or anti-cyclic citrullinated peptide autoantibodies was observed. A meta-analysis of all published STAT4 associations revealed an OR of 1.25 (95% CI 1.19-1.33) (P = 1 x 10(-5)). Conclusion. Our findings indicate an association between the STAT4 polymorphism rs7574865 and RA in 3 different populations, from Spain, Sweden, and The Netherlands, thereby confirming previous data.

AB - Objective. This study was undertaken to investigate the previously reported association of the STAT4 polymorphism rs7574865 with rheumatoid arthritis (RA) in 3 different European populations from Spain, Sweden, and The Netherlands, comprising a total of 2,072 patients and 2,474 controls. Methods. Three different cohorts were included in the study: 923 RA patients and 1,296 healthy controls from Spain, 273 RA patients and 285 healthy controls from. Sweden, and 876 RA patients and 893 healthy controls from The Netherlands. DNA from patients and controls was obtained from peripheral blood. Samples were genotyped for the STAT4 single-nucleotide polymorphism rs7574865 using a TaqMan 5'-allele discrimination assay. The chi-square test was performed to compare allele and genotype distributions. Odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated. Results. We observed a significantly increased frequency of the minor T allele in RA patients compared with healthy controls in the Spanish population (24.8% versus 20.8%; P = 0.001, OR 1.26 [95% CI 1.09-1.45]). This association was confirmed in both the Swedish population (P = 0.03, OR 1.35 [95% CI 1.03-1.77]) and the Dutch population (P = 0.03, OR 1.45 [95% CI 1.21-1.73]). The overall P value for all 3 populations was 9.79 x 10(-6) (OR 1.25 [95% CI 1.13-1.37]). No association between rs7574865 and the presence of rheumatoid factor or anti-cyclic citrullinated peptide autoantibodies was observed. A meta-analysis of all published STAT4 associations revealed an OR of 1.25 (95% CI 1.19-1.33) (P = 1 x 10(-5)). Conclusion. Our findings indicate an association between the STAT4 polymorphism rs7574865 and RA in 3 different populations, from Spain, Sweden, and The Netherlands, thereby confirming previous data.

U2 - 10.1002/art.23549

DO - 10.1002/art.23549

M3 - Article

SN - 1529-0131

VL - 58

SP - 1974

EP - 1980

JO - Arthritis and Rheumatism

JF - Arthritis and Rheumatism

IS - 7

ER -

Association of STAT4 with rheumatoid arthritis - A replication study in three European populations

Abstract

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