At low water activity alpha-chymotrypsin is more active in an ionic liquid than in non-ionic organic solvents

M Eckstein; M Sesing; U Kragl; Patrick Adlercreutz

doi:10.1023/A:1015564608261

At low water activity alpha-chymotrypsin is more active in an ionic liquid than in non-ionic organic solvents

M Eckstein, M Sesing, U Kragl, Patrick Adlercreutz

Research output: Contribution to journal › Article › peer-review

Abstract

The kinetics of the alpha-chymotrypsin catalysed transesterification of N-acetyl-l-phenylalanine ethyl ester with 1-butanol and the competing hydrolysis were evaluated at fixed water activity in two ionic liquids and two non-ionic organic solvents. In most respects the four solvents behaved similarly. However, at a water activity of 0.33, higher catalytic activity was observed in the ionic liquid, 1-butyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl]amide, than in the other solvents, and at a(w)=0.11 catalysis was only observed in this solvent.

Original language	English
Pages (from-to)	867-872
Journal	Biotechnology Letters
Volume	24
Issue number	11
DOIs	https://doi.org/10.1023/A:1015564608261
Publication status	Published - 2002

Subject classification (UKÄ)

Industrial Biotechnology

Free keywords

water activity
alpha-chymotrypsin
ionic liquids

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10.1023/A:1015564608261

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title = "At low water activity alpha-chymotrypsin is more active in an ionic liquid than in non-ionic organic solvents",

abstract = "The kinetics of the alpha-chymotrypsin catalysed transesterification of N-acetyl-l-phenylalanine ethyl ester with 1-butanol and the competing hydrolysis were evaluated at fixed water activity in two ionic liquids and two non-ionic organic solvents. In most respects the four solvents behaved similarly. However, at a water activity of 0.33, higher catalytic activity was observed in the ionic liquid, 1-butyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl]amide, than in the other solvents, and at a(w)=0.11 catalysis was only observed in this solvent.",

keywords = "water activity, alpha-chymotrypsin, ionic liquids",

author = "M Eckstein and M Sesing and U Kragl and Patrick Adlercreutz",

year = "2002",

doi = "10.1023/A:1015564608261",

language = "English",

volume = "24",

pages = "867--872",

journal = "Biotechnology Letters",

issn = "1573-6776",

publisher = "Springer Science and Business Media B.V.",

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TY - JOUR

T1 - At low water activity alpha-chymotrypsin is more active in an ionic liquid than in non-ionic organic solvents

AU - Eckstein, M

AU - Sesing, M

AU - Kragl, U

AU - Adlercreutz, Patrick

PY - 2002

Y1 - 2002

N2 - The kinetics of the alpha-chymotrypsin catalysed transesterification of N-acetyl-l-phenylalanine ethyl ester with 1-butanol and the competing hydrolysis were evaluated at fixed water activity in two ionic liquids and two non-ionic organic solvents. In most respects the four solvents behaved similarly. However, at a water activity of 0.33, higher catalytic activity was observed in the ionic liquid, 1-butyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl]amide, than in the other solvents, and at a(w)=0.11 catalysis was only observed in this solvent.

AB - The kinetics of the alpha-chymotrypsin catalysed transesterification of N-acetyl-l-phenylalanine ethyl ester with 1-butanol and the competing hydrolysis were evaluated at fixed water activity in two ionic liquids and two non-ionic organic solvents. In most respects the four solvents behaved similarly. However, at a water activity of 0.33, higher catalytic activity was observed in the ionic liquid, 1-butyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl]amide, than in the other solvents, and at a(w)=0.11 catalysis was only observed in this solvent.

KW - water activity

KW - alpha-chymotrypsin

KW - ionic liquids

U2 - 10.1023/A:1015564608261

DO - 10.1023/A:1015564608261

M3 - Article

SN - 1573-6776

VL - 24

SP - 867

EP - 872

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 11

ER -

At low water activity alpha-chymotrypsin is more active in an ionic liquid than in non-ionic organic solvents

Abstract

Subject classification (UKÄ)

Free keywords

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