Attovial-based antibody nanoarrays.

Peter Ellmark; Sara Ghatnekar-Nilsson; André Meister; Harry Heinzelmann; Lars Montelius; Christer Wingren; Carl Borrebaeck

doi:10.1002/pmic.200800962

Attovial-based antibody nanoarrays.

Peter Ellmark, Sara Ghatnekar-Nilsson, André Meister, Harry Heinzelmann, Lars Montelius, Christer Wingren, Carl Borrebaeck

Research output: Contribution to journal › Article › peer-review

Abstract

Antibody array-based technology is a powerful emerging tool in proteomics, but to enable global proteome analysis, antibody array layouts with even higher density has to be developed. To this end, we have further developed the first generation of a nanoarray platform, based on attoliter sized vials, attovials, which we have characterized and used for the detection of complement factor C1q in human serum samples. Finally, we demonstrated proof-of-concept for individual functionalisation of the attovials with a recombinant antibody.

Original language	English
Pages (from-to)	5406-5413
Journal	Proteomics
Volume	9
Issue number	24
DOIs	https://doi.org/10.1002/pmic.200800962
Publication status	Published - 2009

Subject classification (UKÄ)

Immunology in the Medical Area (including Cell and Immunotherapy)
Condensed Matter Physics (including Material Physics, Nano Physics)

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10.1002/pmic.200800962

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title = "Attovial-based antibody nanoarrays.",

abstract = "Antibody array-based technology is a powerful emerging tool in proteomics, but to enable global proteome analysis, antibody array layouts with even higher density has to be developed. To this end, we have further developed the first generation of a nanoarray platform, based on attoliter sized vials, attovials, which we have characterized and used for the detection of complement factor C1q in human serum samples. Finally, we demonstrated proof-of-concept for individual functionalisation of the attovials with a recombinant antibody.",

author = "Peter Ellmark and Sara Ghatnekar-Nilsson and Andr{\'e} Meister and Harry Heinzelmann and Lars Montelius and Christer Wingren and Carl Borrebaeck",

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AU - Ellmark, Peter

AU - Ghatnekar-Nilsson, Sara

AU - Meister, André

AU - Heinzelmann, Harry

AU - Montelius, Lars

AU - Wingren, Christer

AU - Borrebaeck, Carl

PY - 2009

Y1 - 2009

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AB - Antibody array-based technology is a powerful emerging tool in proteomics, but to enable global proteome analysis, antibody array layouts with even higher density has to be developed. To this end, we have further developed the first generation of a nanoarray platform, based on attoliter sized vials, attovials, which we have characterized and used for the detection of complement factor C1q in human serum samples. Finally, we demonstrated proof-of-concept for individual functionalisation of the attovials with a recombinant antibody.

U2 - 10.1002/pmic.200800962

DO - 10.1002/pmic.200800962

M3 - Article

SN - 1615-9861

VL - 9

SP - 5406

EP - 5413

JO - Proteomics

JF - Proteomics

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ER -

Attovial-based antibody nanoarrays.

Abstract

Subject classification (UKÄ)

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