Abstract
This study aims to explore a possible silent circulation of wild and vaccine-derived polioviruses in departments of Colombia with polio vaccination coverage of below 80%. The study collected 52 samples of wastewater concentrated as a result of precipitation with polyethylene glycol and sodium chloride. The viral detection was carried out through isolation and the identification through neutralization of the cytopathic effect, as well as through a conventional polymerase chain reaction following reverse transcription. The isolated polioviruses were characterized by the VP1 gene sequence. In two of the 52 samples, there was a presence of the Sabin type 2 poliovirus with more than 99% sequence similarity with the Sabin type 2 strain polio. Circulation of the nonpolio enterovirus was detected in 17.3% of the samples. The serotypes identified corresponded to coxsackievirus B1, echovirus 30, and echovirus 11. No evidence of the spread of either vaccine-derived poliovirus or wild poliovirus was detected in the departments of Colombia with polio coverage lower than 80%.
Translated title of the contribution | Absence of poliovirus circulation in Colombian departments with vaccination coverage below 80% |
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Original language | Spanish |
Pages (from-to) | 156-60 |
Number of pages | 5 |
Journal | Revista panamericana de salud publica = Pan American journal of public health |
Volume | 32 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2012 Aug |
Externally published | Yes |
Subject classification (UKÄ)
- Medical and Health Sciences
Free keywords
- Animals
- Cell Line
- Colombia
- Enterovirus/classification
- Humans
- Mice
- Poliovirus/classification
- Poliovirus Vaccine, Oral
- Serotyping
- Vaccination/statistics & numerical data
- Virus Cultivation
- Waste Water/virology