Azide-Functionalized Naphthoxyloside as a Tool for Glycosaminoglycan Investigations

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We present a xylosylated naphthoxyloside carrying a terminal azide functionality that can be used for conjugation using click chemistry. We show that this naphthoxyloside serves as a substrate for β4GalT7 and induces the formation of soluble glycosaminoglycan (GAG) chains with physiologically relevant lengths and sulfation patterns. Finally, we demonstrate its usefulness by conjugation to the Alexa Fluor 647 and TAMRA fluorophores and coupling to a surface plasmon resonance chip for interaction studies with the hepatocyte growth factor known to interact with the GAG heparan sulfate.

Original languageEnglish
Pages (from-to)2507-2515
JournalBioconjugate Chemistry
Issue number12
Early online date2021
Publication statusPublished - 2021

Subject classification (UKÄ)

  • Biochemistry and Molecular Biology
  • Medical Biotechnology


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