Bacterial Deoxyribonucleoside Kinases Are Poor Suicide Genes in Mammalian Cells

Claire Hebrard, Emeline Cros-Perrial, Anders Ranegaard Clausen, Charles Dumontet, Jure Piskur, Lars Petter Jordheim

Research output: Contribution to journalArticlepeer-review

1 Citation (SciVal)

Abstract

Transfer of deoxyribonucleoside kinases (dNKs) into cancer cells increases the activity of cytotoxic nucleoside analogues. It has been shown that bacterial dNKs, when introduced into Escherichia coli, sensitize this bacterium toward nucleoside analogues. We studied the possibility of using bacterial dNKs, for example deoxyadenosine kinases (dAKs), to sensitize human cancer cells to gemcitabine. Stable and transient transfections of bacterial dNKs into human cells showed that these were much less active than human and fruitfly dNKs. The fusion of dAK from Bacillus cereus to the green fluorescent protein induced a modest sensitization. Apparently, bacterial dNKs did not get properly expressed or are unstable in the mammalian cell.
Original languageEnglish
Pages (from-to)1068-1075
JournalNucleosides, Nucleotides & Nucleic Acids
Volume28
Issue number11-12
DOIs
Publication statusPublished - 2009

Subject classification (UKÄ)

  • Biological Sciences

Keywords

  • bacteria
  • suicide gene
  • gene therapy
  • Deoxyribonucleoside kinases
  • gemcitabine
  • cancer
  • resistance

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