Bacterial ferrochelatase turns human: Tyr13 determines the apparent metal specificity of Bacillus subtilis ferrochelatase.

Mattias Hansson, Tobias Karlberg, Christopher Söderberg, Sreekanth Rajan, Martin J Warren, Salam Al-Karadaghi, Stephen E J Rigby, Mats Hansson

Research output: Contribution to journalArticlepeer-review

8 Citations (SciVal)

Abstract

Ferrochelatase catalyzes the insertion of Fe(2+) into protoporphyrin IX. The enzymatic product heme (protoheme IX) is a well-known cofactor in a wide range of proteins. The insertion of metal ions other than Fe(2+) occurs rarely in vivo, but all ferrochelatases that have been studied can insert Zn(2+) at a good rate in vitro. Co(2+), but not Cu(2+), is known to be a good substrate of the mammalian and Saccharomyces cerevisiae ferrochelatases. In contrast, Cu(2+), but not Co(2+), has been found to be a good substrate of bacterial Bacillus subtilis ferrochelatase. It is not known how ferrochelatase discriminates between different metal ion substrates. Structural analysis of B. subtilis ferrochelatase has shown that Tyr13 is an indirect ligand of Fe(2+) and a direct ligand of a copper mesoporphyrin product. A structure-based comparison revealed that Tyr13 aligns with a Met residue in the S. cerevisiae and human ferrochelatases. Tyr13 was changed to Met in the B. subtilis enzyme by site-directed mutagenesis. Enzymatic measurements showed that the modified enzyme inserted Co(2+) at a higher rate than the wild-type B. subtilis ferrochelatase, but it had lost the ability to use Cu(2+) as a substrate. Thus, the B. subtilis Tyr13Met ferrochelatase showed the same metal specificity as that of the ferrochelatases from S. cerevisiae and human.
Original languageEnglish
Pages (from-to)235-242
JournalJournal of Biological Inorganic Chemistry
Volume16
Issue number2
DOIs
Publication statusPublished - 2011

Subject classification (UKÄ)

  • Biological Sciences

Keywords

  • Site-Directed
  • Mutagenesis
  • Ferrochelatase/*chemistry/genetics/*metabolism
  • Copper/metabolism
  • Cobalt/metabolism
  • Bacillus subtilis/*enzymology
  • Bacterial Proteins/chemistry/genetics/metabolism
  • Structure-Activity Relationship
  • Substrate Specificity
  • Tyrosine/chemistry/genetics/metabolism

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