Bacterial identification by optical mapping of genomic DNA in nanofluidic channels

My Nyblom, Vilhelm Müller, Anna Johnning, Marie Wrande, Albertas Dvirnas, K. K. Sriram, Christian G. Giske, Tobias Ambjörnsson, Linus Sandegren, Erik Kristiansson, Fredrik Westerlund

Research output: Chapter in Book/Report/Conference proceedingPaper in conference proceedingpeer-review

Abstract

A variety of pathogenic bacteria can infect humans and the increase in bacteria resistant to common antibiotics is a large threat to human health worldwide. This work presents a method, based on optical DNA mapping (ODM) in nanofluidic channels, that can detect the type of bacterial present in a sample by matching the obtained maps of large DNA molecules to a database of fully assembled bacterial genomes. The extraction and labelling protocol has been designed to work for both Gram-positive and Gram-negative bacteria, not requiring any prior knowledge about the sample content.

Original languageEnglish
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PublisherChemical and Biological Microsystems Society
Pages821-822
Number of pages2
ISBN (Electronic)9781733419000
Publication statusPublished - 2019
Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland
Duration: 2019 Oct 272019 Oct 31

Publication series

Name23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Country/TerritorySwitzerland
CityBasel
Period2019/10/272019/10/31

Subject classification (UKÄ)

  • Microbiology in the medical area
  • Infectious Medicine
  • Biophysics
  • Other Physics Topics

Keywords

  • Bacterial Identification
  • Nanofluidics
  • Optical DNA Mapping

Fingerprint

Dive into the research topics of 'Bacterial identification by optical mapping of genomic DNA in nanofluidic channels'. Together they form a unique fingerprint.

Cite this