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Abstract
Background: No strawberry allergen has so far been identified and characterized.
Methods: Serum samples were collected from patients with a suggestive case history of adverse reactions to strawberry and other fruits. Extracts from fresh
and frozen strawberries were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting and mass spectrometry. Patient blood samples were analysed for inhibition of IgE binding and basophil degranulation.
Results: Several IgE-binding proteins could be detected. In more than half of the patient sera, a 20/18-kDa doublet band was observed in Western blotting. These
two bands were excised and analysed by mass spectrometry showing the presence of proteins belonging to the Bet v 1 family of allergens. Inhibition of the IgE binding to the 20/18-kDa doublet was obtained by addition of two recombinantly expressed allergens belonging to the Bet v 1 family (Bet v 1 and Mal d 1) and strawberry protein extract. In a cell-based assay of patient blood samples,
basophil degranulation could be induced by strawberry protein extract and by Bet v 1 and Mal d 1.
Conclusions: We conclude that strawberry homologues to Bet v 1 may be allergens of importance for adverse reactions to strawberry.
Methods: Serum samples were collected from patients with a suggestive case history of adverse reactions to strawberry and other fruits. Extracts from fresh
and frozen strawberries were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting and mass spectrometry. Patient blood samples were analysed for inhibition of IgE binding and basophil degranulation.
Results: Several IgE-binding proteins could be detected. In more than half of the patient sera, a 20/18-kDa doublet band was observed in Western blotting. These
two bands were excised and analysed by mass spectrometry showing the presence of proteins belonging to the Bet v 1 family of allergens. Inhibition of the IgE binding to the 20/18-kDa doublet was obtained by addition of two recombinantly expressed allergens belonging to the Bet v 1 family (Bet v 1 and Mal d 1) and strawberry protein extract. In a cell-based assay of patient blood samples,
basophil degranulation could be induced by strawberry protein extract and by Bet v 1 and Mal d 1.
Conclusions: We conclude that strawberry homologues to Bet v 1 may be allergens of importance for adverse reactions to strawberry.
| Original language | English |
|---|---|
| Pages (from-to) | 1277-1284 |
| Journal | Allergy |
| Volume | 59 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2004 |
Subject classification (UKÄ)
- Respiratory Medicine and Allergy
Free keywords
- IgE
- pollen
- food allergy
- allergens
- clinical immunology
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Dive into the research topics of 'Bet v 1 homologues in strawberry identified as IgE-binding proteins and presumptive allergens'. Together they form a unique fingerprint.Research output
- 1 Doctoral Thesis (compilation)
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Proteome Profiling of Strawberry and Characterization of a Bet v 1-Homologous Allergen
Alm, R., 2007, Department of Chemistry, Lund University. 130 p.Research output: Thesis › Doctoral Thesis (compilation)