Bilirubin recognition via molecularly imprinted supermacroporous cryogels

Goezde Baydemir, Nilay Bereli, Muege Andac, Ridvan Say, Igor Galaev, Adil Denizli

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Recent years molecular imprinting has received considerable attention as an excellent and simple approach to recognize small molecules and bioactive substances. The aim of this study is to prepare the bilirubin-imprinted supermacroporous cryogels which can be used for the adsorption of bilirubin from human plasma. N-methacryloyl(L)-tyrosinemethylester (MAT) was chosen as the preorganization monomer. In the first step, bilirubin was complexed with MAT and the bilirubin-imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-tyrosine methylester) [BR-MIP] cryogel was produced by free radical polymerization initiated by N,N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) pair in an ice bath. After that, the template molecules (i.e., bilirubin) were removed from the polymeric structure using sodium carbonate and sodium hydroxide. The maximum bilirubin adsorption amount was 3.6 mg/g polymer. The relative selectivity coefficients of the BR-MIP cryogel for bilirubin/cholesterol and bilirubin/testosterone mixtures were 7.3 and 3.2 times greater than non-imprinted poly(HEMA-MAT) [NIP] cryogel, respectively. The BR-MIP cryogel could be used many times without decreasing bilirubin adsorption amount significantly. Therefore, as a reusable carrier possessing high selectivity, BR-MIP cryogel has a potential candidate as a clinical hemoperfusion material. (C) 2008 Elsevier B.V. All rights reserved.
    Original languageEnglish
    Pages (from-to)33-38
    JournalColloids and Surfaces B: Biointerfaces
    Volume68
    Issue number1
    DOIs
    Publication statusPublished - 2009

    Subject classification (UKÄ)

    • Physical Chemistry

    Free keywords

    • Bilirubin
    • Molecular recognition
    • Cryogels
    • Molecular imprinting
    • Affinity binding

    Fingerprint

    Dive into the research topics of 'Bilirubin recognition via molecularly imprinted supermacroporous cryogels'. Together they form a unique fingerprint.

    Cite this