Abstract
The polyether ionophore salinomycin has recently captured much interest due to its potent activity against multi-drug resistant cancer cells and cancer stem cells. Previous studies have shown that either acylation of the C20 position or esterification/amidation of the C1 carboxylate moiety is beneficial in terms of biological properties. In this paper, we present the first analogs combining such modifications. Evaluation of the anti-proliferative activity against a series of cancer cell lines showed that acylation of the C20 hydroxyl group improves the activity of salinomycin C1 amides but not of the corresponding C1 esters. Importantly, the activity of several of the doubly modified analogs surpasses that of commonly used cytostatic drugs cisplatin and doxorubicin in the LoVo/DX multi-drug resistant cell line. All analogs were tested against primary acute lymphoblastic leukemia cells in standard cell viability assays; three were more potent than salinomycin. Further studies revealed that selected analogs induced characteristics of apoptotic cell death and increased expression of p53. Additionally, using an ex vivo model of breast tumor, tumor cell viability significantly decreased after treatment with salinomycin or its double-modified derivative (3a) in a time-dependent manner. The present findings indicate that double-modified salinomycin derivatives constitute promising lead compounds for targeting various types of cancer.
Original language | English |
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Pages (from-to) | 510-523 |
Number of pages | 14 |
Journal | European Journal of Medicinal Chemistry |
Volume | 156 |
DOIs | |
Publication status | Published - 2018 Aug 5 |
Subject classification (UKÄ)
- Medicinal Chemistry
- Cancer and Oncology
Free keywords
- Acute lymphoblastic leukemia
- Anti-proliferative activity
- Breast cancer
- Ionophore antibiotics
- Regio-selectivity
- Salinomycin