TY - JOUR
T1 - Biological recovery and properties of poly(3-hydroxybutyrate) from Cupriavidus necator H16
AU - Kunasundari, Balakrishnan
AU - Rodriguez Arza, Carlos
AU - Maurer, Frans H J
AU - Murugaiyah, Vikneswaran
AU - Kaur, Gurjeet
AU - Sudesh, Kumar
PY - 2017/1/1
Y1 - 2017/1/1
N2 - In the present study, we observed that laboratory rats readily consumed lyophilized cells of Cupriavidus necator H16 cultivated using palm oil containing 39 wt% poly(3-hydroxybutyrate) [P(3HB)] as sole diet source. The test animals excreted whitish fecal pellets containing 82–97 wt% P(3HB). The remaining impurities could be easily removed by washing the pellets with water and/or low concentrations of detergent, which resulted in P(3HB) granules of a high purity. The molecular masses and thermal properties of P(3HB) obtained by this method were almost similar to P(3HB) extracted from bacterial cells using chloroform. The method reported here is simple and eliminates the need for solvents and strong chemicals, thus resulting in P(3HB) production that is more ecofriendly.
AB - In the present study, we observed that laboratory rats readily consumed lyophilized cells of Cupriavidus necator H16 cultivated using palm oil containing 39 wt% poly(3-hydroxybutyrate) [P(3HB)] as sole diet source. The test animals excreted whitish fecal pellets containing 82–97 wt% P(3HB). The remaining impurities could be easily removed by washing the pellets with water and/or low concentrations of detergent, which resulted in P(3HB) granules of a high purity. The molecular masses and thermal properties of P(3HB) obtained by this method were almost similar to P(3HB) extracted from bacterial cells using chloroform. The method reported here is simple and eliminates the need for solvents and strong chemicals, thus resulting in P(3HB) production that is more ecofriendly.
KW - Cupriavidus necator H16
KW - PHA
KW - Poly(3-hydroxybutyrate)
KW - Recovery
UR - http://www.scopus.com/inward/record.url?scp=84982733906&partnerID=8YFLogxK
U2 - 10.1016/j.seppur.2016.07.043
DO - 10.1016/j.seppur.2016.07.043
M3 - Article
AN - SCOPUS:84982733906
SN - 1383-5866
VL - 172
SP - 1
EP - 6
JO - Separation and Purification Technology
JF - Separation and Purification Technology
ER -