Biomimetic spinning of artificial spider silk from a chimeric minispidroin

Marlene Andersson; Qiupin Jia; Ana Abella; Xiau Yeen Lee; Michael Landreh; Pasi Purhonen; Hans Hebert; Maria Tenje; Carol V. Robinson; Qing Meng; Gustavo R. Plaza; Jan Johansson; Anna Rising

doi:10.1038/nchembio.2269

Biomimetic spinning of artificial spider silk from a chimeric minispidroin

Marlene Andersson, Qiupin Jia, Ana Abella, Xiau Yeen Lee, Michael Landreh, Pasi Purhonen, Hans Hebert, Maria Tenje, Carol V. Robinson, Qing Meng, Gustavo R. Plaza, Jan Johansson, Anna Rising

Department of Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Herein we present a chimeric recombinant spider silk protein (spidroin) whose aqueous solubility equals that of native spider silk dope and a spinning device that is based solely on aqueous buffers, shear forces and lowered pH. The process recapitulates the complex molecular mechanisms that dictate native spider silk spinning and is highly efficient; spidroin from one liter of bacterial shake-flask culture is enough to spin a kilometer of the hitherto toughest as-spun artificial spider silk fiber.

Original language	English
Pages (from-to)	262-264
Number of pages	3
Journal	Nature Chemical Biology
Volume	13
Issue number	3
DOIs	https://doi.org/10.1038/nchembio.2269
Publication status	Published - 2017 Mar 1

Subject classification (UKÄ)

Biomaterials Science

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10.1038/nchembio.2269

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abstract = "Herein we present a chimeric recombinant spider silk protein (spidroin) whose aqueous solubility equals that of native spider silk dope and a spinning device that is based solely on aqueous buffers, shear forces and lowered pH. The process recapitulates the complex molecular mechanisms that dictate native spider silk spinning and is highly efficient; spidroin from one liter of bacterial shake-flask culture is enough to spin a kilometer of the hitherto toughest as-spun artificial spider silk fiber.",

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AU - Andersson, Marlene

AU - Jia, Qiupin

AU - Abella, Ana

AU - Lee, Xiau Yeen

AU - Landreh, Michael

AU - Purhonen, Pasi

AU - Hebert, Hans

AU - Tenje, Maria

AU - Robinson, Carol V.

AU - Meng, Qing

AU - Plaza, Gustavo R.

AU - Johansson, Jan

AU - Rising, Anna

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Herein we present a chimeric recombinant spider silk protein (spidroin) whose aqueous solubility equals that of native spider silk dope and a spinning device that is based solely on aqueous buffers, shear forces and lowered pH. The process recapitulates the complex molecular mechanisms that dictate native spider silk spinning and is highly efficient; spidroin from one liter of bacterial shake-flask culture is enough to spin a kilometer of the hitherto toughest as-spun artificial spider silk fiber.

AB - Herein we present a chimeric recombinant spider silk protein (spidroin) whose aqueous solubility equals that of native spider silk dope and a spinning device that is based solely on aqueous buffers, shear forces and lowered pH. The process recapitulates the complex molecular mechanisms that dictate native spider silk spinning and is highly efficient; spidroin from one liter of bacterial shake-flask culture is enough to spin a kilometer of the hitherto toughest as-spun artificial spider silk fiber.

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DO - 10.1038/nchembio.2269

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VL - 13

SP - 262

EP - 264

JO - Nature Chemical Biology

JF - Nature Chemical Biology

IS - 3

ER -

Biomimetic spinning of artificial spider silk from a chimeric minispidroin

Abstract

Subject classification (UKÄ)

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