Catalytic activity of noncovalent complexes of horse liver alcohol dehydrogenase, NAD+ and polymers, dissolved or suspended in organic solvents

Carmen Virto; Ingemar Svensson; Patrick Adlercreutz; Bo Mattiasson

doi:10.1007/BF00129022

Catalytic activity of noncovalent complexes of horse liver alcohol dehydrogenase, NAD⁺ and polymers, dissolved or suspended in organic solvents

Carmen Virto, Ingemar Svensson, Patrick Adlercreutz, Bo Mattiasson

Research output: Contribution to journal › Article › peer-review

Abstract

Noncovalent complexes were formed by lyophilization of aqueous solutions containing horse liver alcohol dehydrogenase, NAD⁺ and a polymer [ethyl cellulose or poly(vinyl butyral)]. The complexes expressed higher specific catalytic activity in organic solvents as compared to a corresponding amount of enzyme deposited on to Celite or lyophilized enzyme powder. The noncovalent complexes were soluble in toluene. In butyl acetate and methyl t-butyl ether, suspensions of fine particles were formed.

Original language	English
Pages (from-to)	877-882
Number of pages	6
Journal	Biotechnology Letters
Volume	17
Issue number	8
DOIs	https://doi.org/10.1007/BF00129022
Publication status	Published - 1995 Aug 1

Subject classification (UKÄ)

Biocatalysis and Enzyme Technology

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10.1007/BF00129022

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abstract = "Noncovalent complexes were formed by lyophilization of aqueous solutions containing horse liver alcohol dehydrogenase, NAD+ and a polymer [ethyl cellulose or poly(vinyl butyral)]. The complexes expressed higher specific catalytic activity in organic solvents as compared to a corresponding amount of enzyme deposited on to Celite or lyophilized enzyme powder. The noncovalent complexes were soluble in toluene. In butyl acetate and methyl t-butyl ether, suspensions of fine particles were formed.",

author = "Carmen Virto and Ingemar Svensson and Patrick Adlercreutz and Bo Mattiasson",

year = "1995",

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AU - Virto, Carmen

AU - Svensson, Ingemar

AU - Adlercreutz, Patrick

AU - Mattiasson, Bo

PY - 1995/8/1

Y1 - 1995/8/1

N2 - Noncovalent complexes were formed by lyophilization of aqueous solutions containing horse liver alcohol dehydrogenase, NAD+ and a polymer [ethyl cellulose or poly(vinyl butyral)]. The complexes expressed higher specific catalytic activity in organic solvents as compared to a corresponding amount of enzyme deposited on to Celite or lyophilized enzyme powder. The noncovalent complexes were soluble in toluene. In butyl acetate and methyl t-butyl ether, suspensions of fine particles were formed.

AB - Noncovalent complexes were formed by lyophilization of aqueous solutions containing horse liver alcohol dehydrogenase, NAD+ and a polymer [ethyl cellulose or poly(vinyl butyral)]. The complexes expressed higher specific catalytic activity in organic solvents as compared to a corresponding amount of enzyme deposited on to Celite or lyophilized enzyme powder. The noncovalent complexes were soluble in toluene. In butyl acetate and methyl t-butyl ether, suspensions of fine particles were formed.

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DO - 10.1007/BF00129022

M3 - Article

AN - SCOPUS:0029089096

SN - 0141-5492

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JO - Biotechnology Letters

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Catalytic activity of noncovalent complexes of horse liver alcohol dehydrogenase, NAD⁺ and polymers, dissolved or suspended in organic solvents

Abstract

Subject classification (UKÄ)

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