cDC1-derived IL-27 regulates small intestinal CD4+ T cell homeostasis in mice

Fatemeh Ahmadi, Fredrik Junghus, Christian Ashworth, Amanda Lappalainen, Urs Mörbe, Knut Kotarsky, William W Agace

Research output: Contribution to journalArticlepeer-review


The small intestinal lamina propria contains large numbers of IFNγ-producing T helper (Th1) cells that play important roles in intestinal homeostasis and host defense, but the mechanisms underlying their development remain poorly understood. Here, we demonstrate that Th1 cells accumulate in the SI-LP after weaning and are maintained there long term. While both Th17 and Th1 cell accumulation in the SI-LP was microbiota dependent, Th1 cell accumulation uniquely required IL-27 and MHCII expression by cDC1. This reflected a requirement for IL-27 signaling in the priming of Th1 cells rather than for their maintenance once in the mucosa. cDC1-derived IL-27 was essential for maintaining the Th1-Th17 balance within the SI-LP, and in its absence, remaining Th1 cells expressed enhanced levels of Th17 signature genes. In conclusion, we identify cDC1-derived IL-27 as a key regulator of SI-LP Th1-Th17 cell homeostasis.

Original languageEnglish
JournalThe Journal of experimental medicine
Issue number3
Early online date2023 Mar 6
Publication statusPublished - 2023

Subject classification (UKÄ)

  • Immunology

Free keywords

  • Mice
  • Animals
  • CD4-Positive T-Lymphocytes/metabolism
  • Interleukin-27/metabolism
  • Interleukin-17/metabolism
  • Th17 Cells/metabolism
  • Th1 Cells/metabolism
  • Intestinal Mucosa/metabolism
  • Homeostasis


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