Cellulase production of Trichoderma reesei Rut C 30 using steam-pretreated spruce - Hydrolytic potential of cellulases on different substrates

Z Szengyel, Guido Zacchi, A Varga, K Reczey

Research output: Contribution to journalArticlepeer-review

Abstract

Various techniques are available for the conversion of lignocellulosics to fuel ethanol. During the last decade processes based on enzymatic hydrolysis of cellulose have been investigated more extensively, showing good yield on both hardwood and softwood. The cellulase production of a filamentous fungi, Trichoderma reesei Rut C 30, was examined on carbon sources obtained after steam pretreatment of spruce. These materials were washed fibrous steam-pretreated spruce (SPS), and hemicellulose hydrolysate. The hemicellulose hydrolysate contained, besides water-soluble carbohydrates, lignin and sugar degradation products, which were formed during the pretreatment and proved to be inhibitory to microorganisms. Experiments were performed in a 4-L laboratory fermentor. The hydrolytic capacity of the produced enzyme solutions was compared with two commercially available enzyme preparations, Celluclast and Iogen Cellulase, on SPS, washed SPS, and Solka Flee cellulose powder. There was no significant difference among the different enzymes produced by T, reesei Rut C 30. However, the conversion of cellulose using these enzymes was higher than that obtained with Iogen or Celluclast cellulases using steam-pretreated spruce as substrate.
Original languageEnglish
Pages (from-to)679-691
JournalApplied Biochemistry and Biotechnology
Volume84-6
DOIs
Publication statusPublished - 2000

Subject classification (UKÄ)

  • Biochemistry and Molecular Biology

Free keywords

  • cellulase production
  • steam-pretreated spruce
  • enzymatic capacity of
  • cellulases
  • filter paper activity measurement
  • Trichoderma

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