Changes in the profile of simple mucin-type O-glycans and polypeptide GalNAc-transferases in human testis and testicular neoplasms are associated with germ cell maturation and tumour differentiation

E. Rajpert-De Meyts, S. N. Poll, I. Goukasian, C. Jeanneau, A. S. Herlihy, E. P. Bennett, N. E. Skakkebaek, H. Clausen, Aleksander Giwercman, U. Mandel

Research output: Contribution to journalArticlepeer-review

Abstract

Testicular germ cell tumours (TGCT) exhibit remarkable ability to differentiate into virtually all somatic tissue types. In this study, we investigated changes in mucin-type O-glycosylation, which have been associated with somatic cell differentiation and cancer. Expression profile of simple mucin-type O-glycans (Tn, sialyl-Tn, T), histo-blood group H and A variants and six polypeptide Ga1NAc-transferases (T1-4, T6, T11) that control the site and density of O- glycosylation were analysed by immunohistochemistry during human testis development and in TGCT. Normal testis showed a restricted pattern; gonocytes expressed abundant sialyl-Tn and sialyl-T, and adult spermatogonia were devoid of any glycans, whereas spermatocytes and spermatids expressed exclusively glycans Tn and T and the Ga1NAc-T3 isoform. A subset of mature ejaculated spermatozoa expressed an additional glycan sialyl-T. The pattern found in testicular neoplasms recapitulated the developmental order: Pre-invasive carcinoma in situ (CIS) cells and seminoma expressed fetal type sialylated glycans in keeping with their gonocyte-like phenotype. Neither simple mucin-type O-glycans nor GalNAc-transferase isoforms were found in undifferentiated nonseminoma, i.e. embryonal carcinoma, whereas teratomas expressed them all to some extent but in a disorganized manner. We concluded that simple mucin-type O-glycans and their transferases are developmentally regulated in the human testis, with profound changes associated with neoplasia. The restricted O-glycosylation pattern in haploid germ cells suggests a role in their maturation or egg recognition/fertilization warranting further studies in male infertility, whereas the findings in TGCT provide new diagnostic tools and support our hypothesis that testicular cancer is a developmental disease of germ cell differentiation.
Original languageEnglish
Pages (from-to)805-814
JournalVirchows Archiv: an international journal of pathology
Volume451
Issue number4
DOIs
Publication statusPublished - 2007

Subject classification (UKÄ)

  • Urology and Nephrology
  • Obstetrics, Gynecology and Reproductive Medicine

Free keywords

  • mucin-type O-linked glycosylation
  • GalNAc-transferases
  • testicular neoplasm
  • glycosyltransferases
  • carcinoma in situ testis

Fingerprint

Dive into the research topics of 'Changes in the profile of simple mucin-type O-glycans and polypeptide GalNAc-transferases in human testis and testicular neoplasms are associated with germ cell maturation and tumour differentiation'. Together they form a unique fingerprint.

Cite this