Characterisation of a glycosylated alkyl polyglycoside produced by a cyclodextrin glycosyltransferase by HPLC-ELSD and -MS.

David Svensson, Patrick Adlercreutz

Research output: Contribution to journalArticlepeer-review

6 Citations (SciVal)

Abstract

A transglycosylation reaction between an alkyl polyglycoside and α-cyclodextrin catalysed by cyclodextrin glycosyltransferase (CGTase) from Bacillus macerans was investigated. The reaction products were identified by comparison with standards generated by CGTase catalysed modification of pure alkyl glycosides using HPLC-ELSD and -MS analysis. The main products were alkyl glucopyranosides (substrates present in the alkyl polyglycoside) glycosylated with 6 (primary coupling products) or 12 (secondary coupling products) glucose residues. Both α and β anomers were glycosylated.
Original languageEnglish
Pages (from-to)1857-1860
JournalJournal of Chromatography. B
Volume879
DOIs
Publication statusPublished - 2011

Subject classification (UKÄ)

  • Industrial Biotechnology

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