Characterization of a novel testicular form of human hormone-sensitive lipase

A Mairal, N Melaine, H Laurell, J Grober, Lena Stenson, T Guillaudeux, Cecilia Holm, B Jegou, D Langin

Research output: Contribution to journalArticlepeer-review

30 Citations (SciVal)

Abstract

Hormone-sensitive lipase (HSL) is an esterase and lipase, which are essential for spermatogenesis. Two HSL mRNAs are expressed in human testis. A long form is encoded by a testis-specific exon and nine exons common to testis and adipocyte HSL. Here we show that the short-form 3.3-kb mRNA possesses a unique 5' end that is transcribed from a novel testis-specific exon. The corresponding protein is similar to the 775-amino-acid-long adipocyte HSL. Immunohistochemistry experiments on human testis sections revealed that the long form is strictly expressed in haploid germ cells whereas the short form is expressed in interstitial and tubular somatic cells as well as premeiotic germ cells.
Original languageEnglish
Pages (from-to)286-290
JournalBiochemical and Biophysical Research Communications
Volume291
Issue number2
DOIs
Publication statusPublished - 2002

Subject classification (UKÄ)

  • Biological Sciences

Keywords

  • testis
  • spermatogenesis
  • lipid metabolism
  • fertility
  • lipase
  • esterase

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