Characterization of the human CREB3L2 gene promoter

Ioannis Panagopoulos, Fredrik Mertens

Research output: Contribution to journalArticlepeer-review

4 Citations (SciVal)

Abstract

CREB3L2 encodes a member of the CREB3 family of transcription factors. We characterized its promoter region, showing that it is asymmetrically bidirectional, also driving the expression of a variant of AKR1D1. It has a CRE binding site which is conserved among mammalians; removal or alteration of it resulted in reduced promoter activity. When transiently transfecting the HEK293 cell line with constructs with partially deleted promoter regions, 5' deletions beyond 1058-bp upstream of the transcription starting site resulted in successive reduction of the activity. The inclusion of the untranslated part of CREB3L2 exon 1 strongly inhibited the promoter activity. Forskolin resulted in a decreased reporter activity, whereas phorbol 12-myristate 13-acetate increased the promoter activity irrespective of the status of the CRE binding site. The presence of the CRE site indicates autoregulation of CREB3L2 and/or regulation via other members of the CREB3 family or a variety of bZIP transcription factors.

Original languageEnglish
Pages (from-to)615-624
Number of pages10
JournalOncology Reports
Volume21
Issue number3
DOIs
Publication statusPublished - 2009 Mar

Subject classification (UKÄ)

  • Cancer and Oncology
  • Medical Genetics

Keywords

  • Base Sequence
  • Basic-Leucine Zipper Transcription Factors
  • Gene Expression Regulation
  • Humans
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Promoter Regions, Genetic
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Nucleic Acid
  • Journal Article
  • Research Support, Non-U.S. Gov't

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