Chemical characterization of cyanogen bromide fragments from the beta-chain of human complement factor C3

Åke Lundwall; U. Hellman; G. Eggertsen; J. Sjöquist

Chemical characterization of cyanogen bromide fragments from the beta-chain of human complement factor C3

Åke Lundwall, U. Hellman, G. Eggertsen, J. Sjöquist

Uppsala University

Research output: Contribution to journal › Article › peer-review

Abstract

The isolated beta-chain of human complement factor C3 (C3 beta) was fragmented by cyanogen bromide. Nine fragments were defined by gel filtration and high-pressure liquid chromatography, and characterized with respect to their Mr, amino acid composition and N-terminal amino acid sequence. Approx. 30% of the primary structure of C3 beta was determined. Alignment of the 3 N-terminal fragments allowed determination of 61 of the amino terminal residues of C3 beta. This region demonstrated 40% homology with the sequence in the N-terminal segment of the alpha-chain of the cobra venom factor.

Original language	English
Pages (from-to)	57-62
Journal	FEBS Letters
Volume	169
Issue number	1
Publication status	Published - 1984
Externally published	Yes

Bibliographical note

1

Subject classification (UKÄ)

Medicinal Chemistry

Free keywords

*Cyanogen Bromide
Complement C3/*analysis
High Pressure Liquid
Gel
Chromatography
Amino Acid Sequence
Amino Acids/analysis
Humans
Peptide Fragments/*analysis
Research Support
Non-U.S. Gov't

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title = "Chemical characterization of cyanogen bromide fragments from the beta-chain of human complement factor C3",

abstract = "The isolated beta-chain of human complement factor C3 (C3 beta) was fragmented by cyanogen bromide. Nine fragments were defined by gel filtration and high-pressure liquid chromatography, and characterized with respect to their Mr, amino acid composition and N-terminal amino acid sequence. Approx. 30% of the primary structure of C3 beta was determined. Alignment of the 3 N-terminal fragments allowed determination of 61 of the amino terminal residues of C3 beta. This region demonstrated 40% homology with the sequence in the N-terminal segment of the alpha-chain of the cobra venom factor.",

keywords = "*Cyanogen Bromide, Complement C3/*analysis, High Pressure Liquid, Gel, Chromatography, Amino Acid Sequence, Amino Acids/analysis, Humans, Peptide Fragments/*analysis, Research Support, Non-U.S. Gov't",

author = "{\AA}ke Lundwall and U. Hellman and G. Eggertsen and J. Sj{\"o}quist",

note = "1",

year = "1984",

language = "English",

volume = "169",

pages = "57--62",

journal = "FEBS Letters",

issn = "1873-3468",

publisher = "Wiley-Blackwell",

number = "1",

}

TY - JOUR

T1 - Chemical characterization of cyanogen bromide fragments from the beta-chain of human complement factor C3

AU - Lundwall, Åke

AU - Hellman, U.

AU - Eggertsen, G.

AU - Sjöquist, J.

N1 - 1

PY - 1984

Y1 - 1984

N2 - The isolated beta-chain of human complement factor C3 (C3 beta) was fragmented by cyanogen bromide. Nine fragments were defined by gel filtration and high-pressure liquid chromatography, and characterized with respect to their Mr, amino acid composition and N-terminal amino acid sequence. Approx. 30% of the primary structure of C3 beta was determined. Alignment of the 3 N-terminal fragments allowed determination of 61 of the amino terminal residues of C3 beta. This region demonstrated 40% homology with the sequence in the N-terminal segment of the alpha-chain of the cobra venom factor.

AB - The isolated beta-chain of human complement factor C3 (C3 beta) was fragmented by cyanogen bromide. Nine fragments were defined by gel filtration and high-pressure liquid chromatography, and characterized with respect to their Mr, amino acid composition and N-terminal amino acid sequence. Approx. 30% of the primary structure of C3 beta was determined. Alignment of the 3 N-terminal fragments allowed determination of 61 of the amino terminal residues of C3 beta. This region demonstrated 40% homology with the sequence in the N-terminal segment of the alpha-chain of the cobra venom factor.

KW - Cyanogen Bromide

KW - Complement C3/analysis

KW - High Pressure Liquid

KW - Gel

KW - Chromatography

KW - Amino Acid Sequence

KW - Amino Acids/analysis

KW - Humans

KW - Peptide Fragments/analysis

KW - Research Support

KW - Non-U.S. Gov't

M3 - Article

SN - 1873-3468

VL - 169

SP - 57

EP - 62

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Chemical characterization of cyanogen bromide fragments from the beta-chain of human complement factor C3

Abstract

Bibliographical note

Subject classification (UKÄ)

Free keywords

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