Chemical investigations of disperse dyes in patch test preparations.

Kristina Ryberg, Birgitta Gruvberger, Erik Zimerson, Marléne Isaksson, Lena Persson, Östen Sörensen, An Goossens, Magnus Bruze

Research output: Contribution to journalArticlepeer-review


BACKGROUND: Contact allergy to textile dyes is not uncommon. The allergy is detected by patch testing patients with commercial patch test preparations. OBJECTIVE: To investigate 8 disperse dyes (DDs) used for patch testing in the departments in Malmö and in Leuven and to compare them with test preparations used at various dermatology departments. Materials/Methods: The investigated DDs were Disperse Blue (DB) 35, 106, and 124, Disperse Yellow (DY) 3, Disperse Orange (DO) 1 and 3, and Disperse Red (DR) 1 and 17. From 13 clinics, 107 petrolatum preparations were analysed using high-performance liquid chromatography and thin-layer chromatography (TLC), and compared with reference substances obtained at the Malmö laboratory. Concerning DB 35, no reference substance could be identified. RESULTS: TLC visualized impurities in all DDs. For each DD, except DB 35, the mean concentration in the preparations labelled to contain 1.0% (w/w) were DB 106: 0.30%, DB 124: 0.25%, DY 3: 0.44%, DO 1: 0.40%, DO 3: 0.68%, DR 1: 0.49%, and DR 17: 0.35%; there were variations between the samples also with regard to the number of impurities. DO 3 could not be demonstrated in 4/15 preparations labelled DO 3. CONCLUSION: The results may have implications for individual diagnosis and prevention and when comparing test results from various centres.
Original languageEnglish
Pages (from-to)199-209
JournalContact Dermatitis
Issue number4
Publication statusPublished - 2008

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Pediatrics/Urology/Gynecology/Endocrinology (013240400), Occupational and Environmental Dermatology Unit (013241310), Drug Target Discovery (013212045)

Subject classification (UKÄ)

  • Dermatology and Venereal Diseases


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