Research output per year
Research output per year
Abstract
Single-stranded overhangs of the G-rich strand belong to the conserved features of telomeres composed of short telomeric repeats. These structures are thought to be essential for the maintenance of proper telomeric structure and function and the mechanism of their generation is telomerase-independent. We have examined the presence of single-stranded overhangs in Chironomus tentans, a dipteran insect lacking canonical telomeres that uses 350-bp repeats to terminate its chromosomes. Using a non-denaturing in-gel hybridization technique, we found that C. tentans telomeres are unlikely to have single-stranded overhangs longer than 30 nt found in most other higher eukaryotes. These differences might reflect special capping mechanisms for telomeres terminated with long complex repeats.
| Original language | English |
|---|---|
| Pages (from-to) | 21-31 |
| Journal | Chromosome Research |
| Volume | 10 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2002 |
Bibliographical note
The information about affiliations in this record was updated in December 2015.The record was previously connected to the following departments: Genetics (Closed 2011) (011005100), Invertebral Developmental Biology, Udo Haecker's group (013212048)
Subject classification (UKÄ)
- Genetics and Genomics
Free keywords
- Animal
- Chironomidae : genetics
- Chromosomes : ultrastructure
- DNA : genetics
- Nucleic Acid Denaturation
- Exodeoxyribonucleases
- Oligodeoxyribonucleotides
- Telomere : genetics
- Support Non-U.S. Gov't
- Base Sequence
Fingerprint
Dive into the research topics of 'Chromosome ends in Chironomus tentans do not have long single-stranded overhangs characterizing canonical telomeres.'. Together they form a unique fingerprint.Research output
- 1 Doctoral Thesis (compilation)
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Telomeric DNA in Chironomus, a naturally telomerase-free system
Rosén, M., 2001, Department of Genetics, Lund University. 130 p.Research output: Thesis › Doctoral Thesis (compilation)