Chromosome-scale genome assembly of the brown anole (Anolis sagrei), an emerging model species

Anthony J. Geneva, Sungdae Park, Dan G. Bock, Pietro L.H. de Mello, Fatih Sarigol, Marc Tollis, Colin M. Donihue, R. Graham Reynolds, Nathalie Feiner, Ashley M. Rasys, James D. Lauderdale, Sergio G. Minchey, Aaron J. Alcala, Carlos R. Infante, Jason J. Kolbe, Dolph Schluter, Douglas B. Menke, Jonathan B. Losos

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Rapid technological improvements are democratizing access to high quality, chromosome-scale genome assemblies. No longer the domain of only the most highly studied model organisms, now non-traditional and emerging model species can be genome-enabled using a combination of sequencing technologies and assembly software. Consequently, old ideas built on sparse sampling across the tree of life have recently been amended in the face of genomic data drawn from a growing number of high-quality reference genomes. Arguably the most valuable are those long-studied species for which much is already known about their biology; what many term emerging model species. Here, we report a highly complete chromosome-scale genome assembly for the brown anole, Anolis sagrei – a lizard species widely studied across a variety of disciplines and for which a high-quality reference genome was long overdue. This assembly exceeds the vast majority of existing reptile and snake genomes in contiguity (N50 = 253.6 Mb) and annotation completeness. Through the analysis of this genome and population resequence data, we examine the history of repetitive element accumulation, identify the X chromosome, and propose a hypothesis for the evolutionary history of fusions between autosomes and the X that led to the sex chromosomes of A. sagrei.

    Original languageEnglish
    Article number1126
    JournalCommunications Biology
    Volume5
    Issue number1
    DOIs
    Publication statusPublished - 2022

    Subject classification (UKÄ)

    • Evolutionary Biology

    Fingerprint

    Dive into the research topics of 'Chromosome-scale genome assembly of the brown anole (Anolis sagrei), an emerging model species'. Together they form a unique fingerprint.

    Cite this