Clinical Implications of Discrepancy between One-stage Clotting and Chromogenic Factor IX Activity in Haemophilia B

David E. Schmidt, Asa Truedsson, Annelie Cecilia Strålfors, Johanne Andersen Højbjerg, Nida Soutari, Margareta Holmström, Susanna Ranta, Anne Letelier, Annette Bowyer, Rolf Ljung, Jovan P Antovic, Maria Bruzelius

Research output: Contribution to journalArticlepeer-review


BACKGROUND: Discrepancy in factor IX activity (FIX:C) between one-stage assay (OSA) and chromogenic substrate assay (CSA) in patients with haemophilia B (PwHB) introduces challenges for clinical management.

AIM: To study the differences in FIX activity using OSA and CSA in moderate and mild haemophilia B (HB), their impact on classification of severity, and correlation with genotype.

METHODS: Single-centre study including 21 genotyped and clinically characterised PwHB. FIX:C by OSA was measured using ActinFSL (Siemens) and CSA by Biophen (Hyphen). In addition, in-vitro experiments with wild-type FIX were performed. Reproducibility of CSA was assessed between three European coagulation laboratories.

RESULTS: FIX:C by CSA was consistently lower than by OSA, with 10/17 PwHB having a more severe haemophila type by CSA. OSA displayed a more accurate description of the clinical bleeding severity, compared with CSA. A two-fold difference between OSA:CSA FIX:C was present in 12/17 PwHB; all patients had genetic missense variants in the FIX serine protease domain. Discrepancy was also observed with healthy control FIX, most significant for values below 0.10 IU/mL. Assessment of samples with low FIX:C showed excellent reproducibility of the CSA results between the laboratories.

CONCLUSIONS: FIX activity was consistently higher by OSA compared to the CSA. Assessing FIX by CSA alone would have led to diagnosis of a more severe haemophilia type in a significant proportion of patients. Our study suggests using both OSA and CSA FIX:C together with genotyping to classify haemophilia B severity and provide essential information for clinical management.

Original languageEnglish
JournalThrombosis and Haemostasis
Publication statusE-pub ahead of print - 2023 Jul 26

Bibliographical note

Thieme. All rights reserved.

Subject classification (UKÄ)

  • Hematology


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