Cloning and characterization of cDNA encoding a novel human leukotriene B(4) receptor

Ylva Tryselius, Niclas Nilsson, Knut Kotarsky, Björn Olde, Christer Owman

Research output: Contribution to journalArticlepeer-review

55 Citations (SciVal)

Abstract

By homology screening using BLAST searches of expressed sequence tags (ESTs), we have found a previously unidentified cDNA encoding a putative seven-transmembrane receptor with highest similarity to the leukotriene B(4) receptor, BLTR. Analysis of calcium flow in transfected cells, along with sequence analysis, revealed that the EST encoded a functionally inactive protein, lacking the segment corresponding to the C-terminal part of the putative receptor protein. The missing segment was obtained by PCR amplification of a human leukocyte cDNA library and ligated to the truncated EST cDNA. The novel cDNA encodes a full-length receptor with 39% identity to the previously cloned BLTR. Studies of intracellular calcium flow of transfected HeLa cells exposed to various leukotrienes showed that also the novel BLTR-like receptor can be activated by leukotriene B(4), and it is therefore tentatively named BLTR2.
Original languageEnglish
Pages (from-to)377-382
JournalBiochemical and Biophysical Research Communications
Volume274
Issue number2
DOIs
Publication statusPublished - 2000

Subject classification (UKÄ)

  • Biological Sciences

Keywords

  • leukotriene B4
  • BLTR2
  • BLTR
  • calcium assay
  • 7TM receptor

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