TY - JOUR
T1 - Coimmobilization of substrate and biocatalyst
T2 - A method for bioconversion of poorly soluble substances in water milieu
AU - Kaul, Rajni
AU - Adlercreutz, Patrick
AU - Mattiasson, Bo
PY - 1986/1/1
Y1 - 1986/1/1
N2 - Coimmobilization of biocatalyst and substrate was studied as a method to increase the conversion rate in systems with substrates of extremely low solubility in water. The system studied was the conversion of hydrocortisone to prednisolone by Arthrobacter simplex. As a matrix for coimmobilization, alginate turned out to be superior to agar and agarose. After the reaction was complete, the beads were solubilized, andthe cells recovered for reuse, by centrifugation, whereas the prednisolone was extracted from the supernatant.
AB - Coimmobilization of biocatalyst and substrate was studied as a method to increase the conversion rate in systems with substrates of extremely low solubility in water. The system studied was the conversion of hydrocortisone to prednisolone by Arthrobacter simplex. As a matrix for coimmobilization, alginate turned out to be superior to agar and agarose. After the reaction was complete, the beads were solubilized, andthe cells recovered for reuse, by centrifugation, whereas the prednisolone was extracted from the supernatant.
UR - http://www.scopus.com/inward/record.url?scp=0022787937&partnerID=8YFLogxK
U2 - 10.1002/bit.260280917
DO - 10.1002/bit.260280917
M3 - Article
AN - SCOPUS:0022787937
SN - 0006-3592
VL - 28
SP - 1432
EP - 1437
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
IS - 9
ER -