Compact oleic acid in HAMLET.

Jonas Fast; Anki Mossberg; Hanna Nilsson; Catharina Svanborg; Mikael Akke; Sara Linse

doi:10.1016/j.febslet.2005.08.089

Compact oleic acid in HAMLET.

Jonas Fast, Anki Mossberg, Hanna Nilsson, Catharina Svanborg, Mikael Akke, Sara Linse

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Abstract

HAMLET (human alpha-lactalbumin made lethal to tumor cells) is a complex between α-lactalbumin and oleic acid that induces apoptosis in tumor cells, but not in healthy cells. Heteronuclear nuclear magnetic resonance (NMR) spectroscopy was used to determine the structure of 13C-oleic acid in HAMLET, and to study the 15N-labeled protein. Nuclear Overhauser enhancement spectroscopy shows that the two ends of the fatty acid are in close proximity and close to the double bond, indicating that the oleic acid is bound to HAMLET in a compact conformation. The data further show that HAMLET is a partly unfolded/molten globule-like complex under physiological conditions.

Original language	English
Pages (from-to)	6095-6100
Journal	FEBS Letters
Volume	579
Issue number	27
DOIs	https://doi.org/10.1016/j.febslet.2005.08.089
Publication status	Published - 2005

Subject classification (UKÄ)

Biological Sciences

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.febslet.2005.08.089

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title = "Compact oleic acid in HAMLET.",

abstract = "HAMLET (human alpha-lactalbumin made lethal to tumor cells) is a complex between α-lactalbumin and oleic acid that induces apoptosis in tumor cells, but not in healthy cells. Heteronuclear nuclear magnetic resonance (NMR) spectroscopy was used to determine the structure of 13C-oleic acid in HAMLET, and to study the 15N-labeled protein. Nuclear Overhauser enhancement spectroscopy shows that the two ends of the fatty acid are in close proximity and close to the double bond, indicating that the oleic acid is bound to HAMLET in a compact conformation. The data further show that HAMLET is a partly unfolded/molten globule-like complex under physiological conditions.",

author = "Jonas Fast and Anki Mossberg and Hanna Nilsson and Catharina Svanborg and Mikael Akke and Sara Linse",

year = "2005",

doi = "10.1016/j.febslet.2005.08.089",

language = "English",

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journal = "FEBS Letters",

issn = "1873-3468",

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T1 - Compact oleic acid in HAMLET.

AU - Fast, Jonas

AU - Mossberg, Anki

AU - Nilsson, Hanna

AU - Svanborg, Catharina

AU - Akke, Mikael

AU - Linse, Sara

PY - 2005

Y1 - 2005

N2 - HAMLET (human alpha-lactalbumin made lethal to tumor cells) is a complex between α-lactalbumin and oleic acid that induces apoptosis in tumor cells, but not in healthy cells. Heteronuclear nuclear magnetic resonance (NMR) spectroscopy was used to determine the structure of 13C-oleic acid in HAMLET, and to study the 15N-labeled protein. Nuclear Overhauser enhancement spectroscopy shows that the two ends of the fatty acid are in close proximity and close to the double bond, indicating that the oleic acid is bound to HAMLET in a compact conformation. The data further show that HAMLET is a partly unfolded/molten globule-like complex under physiological conditions.

AB - HAMLET (human alpha-lactalbumin made lethal to tumor cells) is a complex between α-lactalbumin and oleic acid that induces apoptosis in tumor cells, but not in healthy cells. Heteronuclear nuclear magnetic resonance (NMR) spectroscopy was used to determine the structure of 13C-oleic acid in HAMLET, and to study the 15N-labeled protein. Nuclear Overhauser enhancement spectroscopy shows that the two ends of the fatty acid are in close proximity and close to the double bond, indicating that the oleic acid is bound to HAMLET in a compact conformation. The data further show that HAMLET is a partly unfolded/molten globule-like complex under physiological conditions.

U2 - 10.1016/j.febslet.2005.08.089

DO - 10.1016/j.febslet.2005.08.089

M3 - Article

SN - 1873-3468

VL - 579

SP - 6095

EP - 6100

JO - FEBS Letters

JF - FEBS Letters

IS - 27

ER -

Compact oleic acid in HAMLET.

Abstract

Subject classification (UKÄ)

UN SDGs

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