Comparative immunomodulatory effects in mice and in human dendritic cells of five bacterial strains selected for biocontrol of leafy green vegetables

Elisabeth Uhlig, Giulia Elli, Noora Nurminen, Elin Oscarsson, Pamela Canaviri-Paz, Stina Burri, Anne-Marie Rohrstock, Milladur Rahman, Beatrix Alsanius, Göran Molin, Kathrin Stephanie Zeller, Åsa Håkansson

Research output: Contribution to journalArticlepeer-review

Abstract

The market for ready-to eat vegetables is increasing, but unfortunately so do the numbers of food-borne illness outbreaks related to these products. A previous study has identified bacterial strains suitable for biocontrol of leafy green vegetables to reduce the exposure to pathogens in these products. As a tentative safety evaluation, five selected strains (Rhodococcus cerastii MR5x, Bacillus coagulans LMG P-32205, Bacillus coagulans LMG P-32206, Pseudomonas cedrina LMG P-32207 and Pseudomonas punonensis LMG P-32204) were individually compared for immunomodulating effects in mice and in human monocyte-derived dendritic cells (MoDCs). Mice receiving the two B. coagulans strains consistently resemble the immunological response of the normal control, and no, or low, cell activation and pro-inflammatory cytokine expression was observed in MoDCs exposed to B. coagulans strains. However, different responses were seen in the two models for the Gram-negative P. cedrina and the Gram-positive R. cerastii. Moreover, P. punonensis and B. coagulans increased the microbiota diversity in mice as seen by the Shannon-Wiener index. In conclusion, the two strains of B. coagulans showed an immunological response that indicate that they lack pathogenic abilities, thus encouraging further safety evaluation and showing great potential to be used as biocontrol agents on leafy green vegetables.

Original languageEnglish
Article number113064
JournalFood and chemical toxicology : an international journal published for the British Industrial Biological Research Association
Volume165
Early online date2022 May 10
DOIs
Publication statusPublished - 2022

Subject classification (UKÄ)

  • Food Science

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