Comparison of (GTG)(5)-oligonucleotide and ribosomal intergenic transcribed spacer (ITS)-PCR for molecular typing of Klebsiella isolates

Anna Ryberg, Crister Olsson, Siv Ahrné, Hans-Juerg Monstein

Research output: Contribution to journalArticlepeer-review

11 Citations (SciVal)

Abstract

Molecular typing of Klebsiella species has become important for monitoring dissemination of beta-lactamase-producers in hospital environments. The present study was designed to evaluate poly-trinucleotide (GTG)(5)- and rDNA intergenic transcribed spacer (ITS)-PCR fingerprint analysis for typing of Klebsiella pneumoniae and Klebsiella oxytoca isolates. Multiple displacement amplified DNA derived from 19K pneumoniae (some with an ESBL-phenotype). 35 K. oxytoca isolates, five K pneumoniae, two K oxytoca, three Raoultella, and one Enterobacter aerogenes type and reference strains underwent (GTG)(5) and ITS-PCR analysis. Dendrograms were constructed using cosine coefficient and the Neighbour joining method. (GTG)(5) and ITS-PCR analysis revealed that K pneumoniae and K oxytoca isolates. reference and type strains formed distinct cluster groups, and tentative subclusters could be established. We conclude that (GTG)(5) and ITS-PCR analysis combined with automated capillary electrophoresis provides promising tools for molecular typing of Klebsiella isolates. (C) 2010 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)183-188
JournalJournal of Microbiological Methods
Volume84
Issue number2
DOIs
Publication statusPublished - 2011

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Applied Nutrition and Food Chemistry (011001300), Surgery Research Unit (013242220)

Subject classification (UKÄ)

  • Surgery

Keywords

  • Klebsiella spp.
  • Molecular typing
  • (GTG)(5)-PCR
  • Ribosomal intergenic
  • transcribed spacer (ITS)-PCR
  • Fingerprint analysis
  • Multiple
  • displacement amplification

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