TY - UNPB
T1 - Comparison of histological delineations of medial temporal lobe cortices by four independent neuroanatomy laboratories
AU - Wuestefeld, Anika
AU - Baumeister, Hannah
AU - Adams, Jenna N
AU - de Flores, Robin
AU - Hodgetts, Carl
AU - Mazloum-Farzaghi, Negar
AU - Olsen, Rosanna K
AU - Puliyadi, Vyash
AU - Tran, Tammy T
AU - Bakker, Arnold
AU - Canada, Kelsey L
AU - Dalton, Marshall A
AU - Daugherty, Ana M
AU - Joie, Renaud La
AU - Wang, Lei
AU - Bedard, Madigan
AU - Buendia, Esther
AU - Denning, Amanda
AU - Irwin, David J
AU - Ittyerah, Ranjit
AU - Lee, Edward B
AU - Lim, Sydney
AU - de Onzoño Martin, Maria Mercedes Iñiguez
AU - Lopez, Monica Munoz
AU - Schuck, Theresa
AU - Trotman, Winifred
AU - Vela, Alicia
AU - Yushkevich, Paul
AU - Amunts, Katrin
AU - Augustinack, Jean C
AU - Ding, Song-Lin
AU - Insausti, Ricardo
AU - Kedo, Olga
AU - Berron, David
AU - Wisse, Laura E M
PY - 2023/5/24
Y1 - 2023/5/24
N2 - The medial temporal lobe (MTL) cortex, located adjacent to the hippocampus, is crucial for memory and prone to the accumulation of certain neuropathologies such as Alzheimer's disease neurofibrillary tau tangles. The MTL cortex is composed of several subregions which differ in their functional and cytoarchitectonic features. As neuroanatomical schools rely on different cytoarchitectonic definitions of these subregions, it is unclear to what extent their delineations of MTL cortex subregions overlap. Here, we provide an overview of cytoarchitectonic definitions of the cortices that make up the parahippocampal gyrus (entorhinal and parahippocampal cortices) and the adjacent Brodmann areas (BA) 35 and 36, as provided by four neuroanatomists from different laboratories, aiming to identify the rationale for overlapping and diverging delineations. Nissl-stained series were acquired from the temporal lobes of three human specimens (two right and one left hemisphere). Slices (50 µm thick) were prepared perpendicular to the long axis of the hippocampus spanning the entire longitudinal extent of the MTL cortex. Four neuroanatomists annotated MTL cortex subregions on digitized (20X resolution) slices with 5 mm spacing. Parcellations, terminology, and border placement were compared among neuroanatomists. Cytoarchitectonic features of each subregion are described in detail. Qualitative analysis of the annotations showed higher agreement in the definitions of the entorhinal cortex and BA35, while definitions of BA36 and the parahippocampal cortex exhibited less overlap among neuroanatomists. The degree of overlap of cytoarchitectonic definitions was partially reflected in the neuroanatomists' agreement on the respective delineations. Lower agreement in annotations was observed in transitional zones between structures where seminal cytoarchitectonic features are expressed more gradually. The results highlight that definitions and parcellations of the MTL cortex differ among neuroanatomical schools and thereby increase understanding of why these differences may arise. This work sets a crucial foundation to further advance anatomically-informed human neuroimaging research on the MTL cortex.
AB - The medial temporal lobe (MTL) cortex, located adjacent to the hippocampus, is crucial for memory and prone to the accumulation of certain neuropathologies such as Alzheimer's disease neurofibrillary tau tangles. The MTL cortex is composed of several subregions which differ in their functional and cytoarchitectonic features. As neuroanatomical schools rely on different cytoarchitectonic definitions of these subregions, it is unclear to what extent their delineations of MTL cortex subregions overlap. Here, we provide an overview of cytoarchitectonic definitions of the cortices that make up the parahippocampal gyrus (entorhinal and parahippocampal cortices) and the adjacent Brodmann areas (BA) 35 and 36, as provided by four neuroanatomists from different laboratories, aiming to identify the rationale for overlapping and diverging delineations. Nissl-stained series were acquired from the temporal lobes of three human specimens (two right and one left hemisphere). Slices (50 µm thick) were prepared perpendicular to the long axis of the hippocampus spanning the entire longitudinal extent of the MTL cortex. Four neuroanatomists annotated MTL cortex subregions on digitized (20X resolution) slices with 5 mm spacing. Parcellations, terminology, and border placement were compared among neuroanatomists. Cytoarchitectonic features of each subregion are described in detail. Qualitative analysis of the annotations showed higher agreement in the definitions of the entorhinal cortex and BA35, while definitions of BA36 and the parahippocampal cortex exhibited less overlap among neuroanatomists. The degree of overlap of cytoarchitectonic definitions was partially reflected in the neuroanatomists' agreement on the respective delineations. Lower agreement in annotations was observed in transitional zones between structures where seminal cytoarchitectonic features are expressed more gradually. The results highlight that definitions and parcellations of the MTL cortex differ among neuroanatomical schools and thereby increase understanding of why these differences may arise. This work sets a crucial foundation to further advance anatomically-informed human neuroimaging research on the MTL cortex.
U2 - 10.1101/2023.05.24.542054
DO - 10.1101/2023.05.24.542054
M3 - Preprint (in preprint archive)
C2 - 37292729
BT - Comparison of histological delineations of medial temporal lobe cortices by four independent neuroanatomy laboratories
PB - bioRxiv
ER -