Comparison of Radioimmunoprecipitation With Luciferase Immunoprecipitation for Autoantibodies to GAD65 and IA-2 beta

Peter D. Burbelo; Hiroki Hirai; Alexandra T. Issa; Albert Kingman; Åke Lernmark; Sten Ivarsson; Abner L. Notkins; Michael J. Iadarola

doi:10.2337/dc09-1938

Comparison of Radioimmunoprecipitation With Luciferase Immunoprecipitation for Autoantibodies to GAD65 and IA-2 beta

Peter D. Burbelo, Hiroki Hirai, Alexandra T. Issa, Albert Kingman, Åke Lernmark, Sten Ivarsson, Abner L. Notkins, Michael J. Iadarola

Research output: Contribution to journal › Article › peer-review

Abstract

OBJECTIVE - To compare the sensitivity and specificity of luciferase immunoprecipitation (LIPS) with radioimmunoprecipitation (RIP) for the measurement of autoantibodies to the type 1 diabetes autoantigens glutamic acid decarboxylase 65 (GAD65) and insulinoma-associated protein (IA)-2 beta. RESEARCH DESIGN AND METHODS - Sera from 49 type 1 diabetic patients and 100 nondiabetic control subjects from Diabetes Antibody Standardization Program 2007 were used to screen for autoantibodies to GAD65. An additional 200 type 1 diabetic patients and 200 nondiabetic control subjects were used to validate the GAD65 results and screen for autoantibodies to IA-2 beta. RESULTS - LIPS showed equal sensitivity and specificity to RIP for detecting autoantibodies to GAD65 and IA-2 beta. Receiver-operating characteristic analysis revealed that the detection of autoantibodies to GAD65 and IA-2 beta by LIPS and RIP were not statistically different. CONCLUSIONS - The LIPS assay does not require the use of radioisotopes or in vitro transcription/translation and is a practical alternative at the clinical level for the RIP assay.

Original language	English
Pages (from-to)	754-756
Journal	Diabetes Care
Volume	33
Issue number	4
DOIs	https://doi.org/10.2337/dc09-1938
Publication status	Published - 2010

Subject classification (UKÄ)

Endocrinology and Diabetes

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2337/dc09-1938

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@article{e6a55e8ec6ea40dfa3d9dae69d8524bf,

title = "Comparison of Radioimmunoprecipitation With Luciferase Immunoprecipitation for Autoantibodies to GAD65 and IA-2 beta",

abstract = "OBJECTIVE - To compare the sensitivity and specificity of luciferase immunoprecipitation (LIPS) with radioimmunoprecipitation (RIP) for the measurement of autoantibodies to the type 1 diabetes autoantigens glutamic acid decarboxylase 65 (GAD65) and insulinoma-associated protein (IA)-2 beta. RESEARCH DESIGN AND METHODS - Sera from 49 type 1 diabetic patients and 100 nondiabetic control subjects from Diabetes Antibody Standardization Program 2007 were used to screen for autoantibodies to GAD65. An additional 200 type 1 diabetic patients and 200 nondiabetic control subjects were used to validate the GAD65 results and screen for autoantibodies to IA-2 beta. RESULTS - LIPS showed equal sensitivity and specificity to RIP for detecting autoantibodies to GAD65 and IA-2 beta. Receiver-operating characteristic analysis revealed that the detection of autoantibodies to GAD65 and IA-2 beta by LIPS and RIP were not statistically different. CONCLUSIONS - The LIPS assay does not require the use of radioisotopes or in vitro transcription/translation and is a practical alternative at the clinical level for the RIP assay.",

author = "Burbelo, {Peter D.} and Hiroki Hirai and Issa, {Alexandra T.} and Albert Kingman and {\AA}ke Lernmark and Sten Ivarsson and Notkins, {Abner L.} and Iadarola, {Michael J.}",

year = "2010",

doi = "10.2337/dc09-1938",

language = "English",

volume = "33",

pages = "754--756",

journal = "Diabetes Care",

issn = "1935-5548",

publisher = "American Diabetes Association",

number = "4",

}

TY - JOUR

T1 - Comparison of Radioimmunoprecipitation With Luciferase Immunoprecipitation for Autoantibodies to GAD65 and IA-2 beta

AU - Burbelo, Peter D.

AU - Hirai, Hiroki

AU - Issa, Alexandra T.

AU - Kingman, Albert

AU - Lernmark, Åke

AU - Ivarsson, Sten

AU - Notkins, Abner L.

AU - Iadarola, Michael J.

PY - 2010

Y1 - 2010

N2 - OBJECTIVE - To compare the sensitivity and specificity of luciferase immunoprecipitation (LIPS) with radioimmunoprecipitation (RIP) for the measurement of autoantibodies to the type 1 diabetes autoantigens glutamic acid decarboxylase 65 (GAD65) and insulinoma-associated protein (IA)-2 beta. RESEARCH DESIGN AND METHODS - Sera from 49 type 1 diabetic patients and 100 nondiabetic control subjects from Diabetes Antibody Standardization Program 2007 were used to screen for autoantibodies to GAD65. An additional 200 type 1 diabetic patients and 200 nondiabetic control subjects were used to validate the GAD65 results and screen for autoantibodies to IA-2 beta. RESULTS - LIPS showed equal sensitivity and specificity to RIP for detecting autoantibodies to GAD65 and IA-2 beta. Receiver-operating characteristic analysis revealed that the detection of autoantibodies to GAD65 and IA-2 beta by LIPS and RIP were not statistically different. CONCLUSIONS - The LIPS assay does not require the use of radioisotopes or in vitro transcription/translation and is a practical alternative at the clinical level for the RIP assay.

AB - OBJECTIVE - To compare the sensitivity and specificity of luciferase immunoprecipitation (LIPS) with radioimmunoprecipitation (RIP) for the measurement of autoantibodies to the type 1 diabetes autoantigens glutamic acid decarboxylase 65 (GAD65) and insulinoma-associated protein (IA)-2 beta. RESEARCH DESIGN AND METHODS - Sera from 49 type 1 diabetic patients and 100 nondiabetic control subjects from Diabetes Antibody Standardization Program 2007 were used to screen for autoantibodies to GAD65. An additional 200 type 1 diabetic patients and 200 nondiabetic control subjects were used to validate the GAD65 results and screen for autoantibodies to IA-2 beta. RESULTS - LIPS showed equal sensitivity and specificity to RIP for detecting autoantibodies to GAD65 and IA-2 beta. Receiver-operating characteristic analysis revealed that the detection of autoantibodies to GAD65 and IA-2 beta by LIPS and RIP were not statistically different. CONCLUSIONS - The LIPS assay does not require the use of radioisotopes or in vitro transcription/translation and is a practical alternative at the clinical level for the RIP assay.

U2 - 10.2337/dc09-1938

DO - 10.2337/dc09-1938

M3 - Article

C2 - 20086252

SN - 1935-5548

VL - 33

SP - 754

EP - 756

JO - Diabetes Care

JF - Diabetes Care

IS - 4

ER -

Comparison of Radioimmunoprecipitation With Luciferase Immunoprecipitation for Autoantibodies to GAD65 and IA-2 beta

Abstract

Subject classification (UKÄ)

UN SDGs

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