Comparison of the prevalence of glutamic acid decarboxylase (GAD65) and gliadin antibodies (AGA) in a randomly selected adult estonian population

R Uibo; E P Sullivan; O Uibo; A Lernmark; L Salur; T Kivik; M Mandel

doi:10.1055/s-2001-17215

Comparison of the prevalence of glutamic acid decarboxylase (GAD65) and gliadin antibodies (AGA) in a randomly selected adult estonian population

R Uibo, E P Sullivan, O Uibo, A Lernmark, L Salur, T Kivik, M Mandel

University of Washington, Seattle

Research output: Contribution to journal › Article › peer-review

Abstract

We aimed to test the hypothesis that gluten might be associated with the development of islet cell autoimmunity. A random sample of 200 persons (87 males, mean age 42.4 years) from Estonia including one patient with type I diabetes mellitus was studied. IgG-type glutamic acid decarboxylase (GAD65) antibodies were determined using radioligand-binding assay and IgG/IgA-type gliadin antibodies (AGA) by enzyme-linked immunosorbent assay. Generic HLA-DRB1* alleles were analyzed using a polymerase chain reaction. Although our results revealed the highest GAD65Ab index and a high IgA-type AGA in a person with diabetes, no correlation between GAD65Ab and AGA values was revealed among the other 199 persons (p > 0.05). There were also no differences between test values among persons with and without different HLA-DRB1* alleles (p > 0.05). In the GAD65Ab assay, one person (0.5 %; 95 % CI: 0 - 1.5) out of 199 exceeded the 99(th) centile of the GAD65Ab index. In summary, the present study does not confirm the possibility that there is a relationship between the immune reactivity against GAD65 and gliadin, at least in persons without type I DM.

Original language	English
Pages (from-to)	564-7
Journal	Hormone and Metabolic Research
Volume	33
Issue number	9
DOIs	https://doi.org/10.1055/s-2001-17215
Publication status	Published - 2001
Externally published	Yes

Free keywords

Adolescent
Adult
Aged
Alleles
Autoantibodies/blood
Diabetes Mellitus, Type 1/immunology
Enzyme-Linked Immunosorbent Assay
Estonia
Female
Gliadin/immunology
Glutamate Decarboxylase/immunology
HLA-DR Antigens/genetics
HLA-DRB1 Chains
Humans
Immunoglobulin A/blood
Immunoglobulin G/blood
Islets of Langerhans/immunology
Male
Middle Aged

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1055/s-2001-17215

Cite this

@article{3c97820b2fb84875b1f44db0c20b5a1a,

title = "Comparison of the prevalence of glutamic acid decarboxylase (GAD65) and gliadin antibodies (AGA) in a randomly selected adult estonian population",

abstract = "We aimed to test the hypothesis that gluten might be associated with the development of islet cell autoimmunity. A random sample of 200 persons (87 males, mean age 42.4 years) from Estonia including one patient with type I diabetes mellitus was studied. IgG-type glutamic acid decarboxylase (GAD65) antibodies were determined using radioligand-binding assay and IgG/IgA-type gliadin antibodies (AGA) by enzyme-linked immunosorbent assay. Generic HLA-DRB1* alleles were analyzed using a polymerase chain reaction. Although our results revealed the highest GAD65Ab index and a high IgA-type AGA in a person with diabetes, no correlation between GAD65Ab and AGA values was revealed among the other 199 persons (p > 0.05). There were also no differences between test values among persons with and without different HLA-DRB1* alleles (p > 0.05). In the GAD65Ab assay, one person (0.5 %; 95 % CI: 0 - 1.5) out of 199 exceeded the 99(th) centile of the GAD65Ab index. In summary, the present study does not confirm the possibility that there is a relationship between the immune reactivity against GAD65 and gliadin, at least in persons without type I DM.",

keywords = "Adolescent, Adult, Aged, Alleles, Autoantibodies/blood, Diabetes Mellitus, Type 1/immunology, Enzyme-Linked Immunosorbent Assay, Estonia, Female, Gliadin/immunology, Glutamate Decarboxylase/immunology, HLA-DR Antigens/genetics, HLA-DRB1 Chains, Humans, Immunoglobulin A/blood, Immunoglobulin G/blood, Islets of Langerhans/immunology, Male, Middle Aged",

author = "R Uibo and Sullivan, {E P} and O Uibo and A Lernmark and L Salur and T Kivik and M Mandel",

year = "2001",

doi = "10.1055/s-2001-17215",

language = "English",

volume = "33",

pages = "564--7",

journal = "Hormone and Metabolic Research",

issn = "0018-5043",

publisher = "Georg Thieme Verlag",

number = "9",

}

TY - JOUR

T1 - Comparison of the prevalence of glutamic acid decarboxylase (GAD65) and gliadin antibodies (AGA) in a randomly selected adult estonian population

AU - Uibo, R

AU - Sullivan, E P

AU - Uibo, O

AU - Lernmark, A

AU - Salur, L

AU - Kivik, T

AU - Mandel, M

PY - 2001

Y1 - 2001

N2 - We aimed to test the hypothesis that gluten might be associated with the development of islet cell autoimmunity. A random sample of 200 persons (87 males, mean age 42.4 years) from Estonia including one patient with type I diabetes mellitus was studied. IgG-type glutamic acid decarboxylase (GAD65) antibodies were determined using radioligand-binding assay and IgG/IgA-type gliadin antibodies (AGA) by enzyme-linked immunosorbent assay. Generic HLA-DRB1* alleles were analyzed using a polymerase chain reaction. Although our results revealed the highest GAD65Ab index and a high IgA-type AGA in a person with diabetes, no correlation between GAD65Ab and AGA values was revealed among the other 199 persons (p > 0.05). There were also no differences between test values among persons with and without different HLA-DRB1* alleles (p > 0.05). In the GAD65Ab assay, one person (0.5 %; 95 % CI: 0 - 1.5) out of 199 exceeded the 99(th) centile of the GAD65Ab index. In summary, the present study does not confirm the possibility that there is a relationship between the immune reactivity against GAD65 and gliadin, at least in persons without type I DM.

AB - We aimed to test the hypothesis that gluten might be associated with the development of islet cell autoimmunity. A random sample of 200 persons (87 males, mean age 42.4 years) from Estonia including one patient with type I diabetes mellitus was studied. IgG-type glutamic acid decarboxylase (GAD65) antibodies were determined using radioligand-binding assay and IgG/IgA-type gliadin antibodies (AGA) by enzyme-linked immunosorbent assay. Generic HLA-DRB1* alleles were analyzed using a polymerase chain reaction. Although our results revealed the highest GAD65Ab index and a high IgA-type AGA in a person with diabetes, no correlation between GAD65Ab and AGA values was revealed among the other 199 persons (p > 0.05). There were also no differences between test values among persons with and without different HLA-DRB1* alleles (p > 0.05). In the GAD65Ab assay, one person (0.5 %; 95 % CI: 0 - 1.5) out of 199 exceeded the 99(th) centile of the GAD65Ab index. In summary, the present study does not confirm the possibility that there is a relationship between the immune reactivity against GAD65 and gliadin, at least in persons without type I DM.

KW - Adolescent

KW - Adult

KW - Aged

KW - Alleles

KW - Autoantibodies/blood

KW - Diabetes Mellitus, Type 1/immunology

KW - Enzyme-Linked Immunosorbent Assay

KW - Estonia

KW - Female

KW - Gliadin/immunology

KW - Glutamate Decarboxylase/immunology

KW - HLA-DR Antigens/genetics

KW - HLA-DRB1 Chains

KW - Humans

KW - Immunoglobulin A/blood

KW - Immunoglobulin G/blood

KW - Islets of Langerhans/immunology

KW - Male

KW - Middle Aged

U2 - 10.1055/s-2001-17215

DO - 10.1055/s-2001-17215

M3 - Article

C2 - 11561218

SN - 0018-5043

VL - 33

SP - 564

EP - 567

JO - Hormone and Metabolic Research

JF - Hormone and Metabolic Research

IS - 9

ER -

Comparison of the prevalence of glutamic acid decarboxylase (GAD65) and gliadin antibodies (AGA) in a randomly selected adult estonian population

Abstract

Free keywords

UN SDGs

Access to Document

Fingerprint

Cite this