Competitive Na+ and Rb+ binding in the minor groove of DNA

Flaminia Cesare Marincola, Vladimir Denisov, Bertil Halle

Research output: Contribution to journalArticlepeer-review

73 Citations (SciVal)

Abstract

Sequence-dependent coordination of alkali ions to the nucleotide bases in the minor groove of AT-tract B-DNA has recently been inferred from X-ray crystallography, solution NMR and computer simulations. Here, we present new Na-23 and Rb-87 magnetic relaxation dispersion (MRD) data that demonstrate competitive and long-lived binding of Na+ and Rb+ ions in the minor groove of the B-DNA duplex [d(CGCGAATTCGCG)](2). The Na+/Rb+ selectivity of the minor groove is found to be weak, consistent with local structural flexibility. The ion occupancies derived from the MRD data are substantially higher than previously reported, suggesting that groove-bound ions significantly influence the energetics and structural polymorphism of DNA in vivo. For example, in the presence of 0.20 M Na+ and 0.56 M Rb+ at 4 degreesC, the ApT site in the minor groove is occupied by a Rb+ ion, a Na+ ion, or a water molecule 40, 10, and 50% of the time, respectively. In the absence of Rb+, the Na+ occupancy increases to 50%. At 4 degreesC, the mean residence time of groove-bound ions is 0.2 +/- 0.1 mus for Rb+ and 10 ns to 100 mus for Na+. A shorter correlation time of 2 ns is attributed to counterions bridging cross-strand phosphate groups.
Original languageEnglish
Pages (from-to)6739-6750
JournalJournal of the American Chemical Society
Volume126
Issue number21
DOIs
Publication statusPublished - 2004

Subject classification (UKÄ)

  • Physical Chemistry

Fingerprint

Dive into the research topics of 'Competitive Na+ and Rb+ binding in the minor groove of DNA'. Together they form a unique fingerprint.

Cite this