Composite Materials in Molecular Imprinting Technology

Ecevit Yilmaz

Research output: ThesisDoctoral Thesis (compilation)


Molecularly imprinted polymers (MIPs) are artificial plastic materials that mimic antibodies or enzymes. Like natural antibodies or enzymes, molecularly imprinted polymers can selectively bind target molecules or can promote catalytic reactions. In contrast to these natural entities, they are robust materials that resist heat, harsh chemicals and the presence of organic solvents. The starting materials are cheap, the preparation is relatively simple and polymer particles have long shelf lives. MIPs constitute therefore promising alternatives to antibodies or enzymes in certain processes. For preparing a MIP, a target molecule along with functional and cross-linking monomers is dissolved in a solvent. The functional monomers interact with the target molecule and form an assembly, which is then frozen in by polymerization. The so obtained polymer block is ground and sieved to yield particles with a desired size. Then the target molecule can be extracted from the polymer particles thereby liberating the specific binding sites. Such traditional, molecularly imprinted polymer particles suffer from a series of disadvantages; the process is quite time consuming and leads to irregular polymer granules that exhibit low capacities, polyclonal binding sites and binding sites that are not well accessible.

This thesis gives an overview of various methods with which molecularly imprinted polymers can be prepared that aim at improving the quality of the recognition site. These include new polymerization methods, construction of binding sites on surfaces and preparation of MIPs utilizing heterogeneous systems like imprinting at interfaces. A focus was on the development of a novel approach to molecular imprinting, with the target molecule covalently immobilized on a solid support material. This thesis also describes methods that yield spherical and monodisperse MIP beads, and methods that lead to other well defined physical forms of MIPs, such as composites with pre-formed beads, composites with porous membranes and thin films on flat substrates.
Original languageEnglish
Awarding Institution
  • Pure and Applied Biochemistry
  • [unknown], [unknown], Supervisor, External person
Award date2002 Sep 13
Print ISBNs91-628-5347-3
Publication statusPublished - 2002

Bibliographical note

Defence details

Date: 2002-09-13
Time: 13:15
Place: Lecture Hall B, Chemical Center

External reviewer(s)

Name: Takeuchi, Toshifumi
Title: Prof
Affiliation: Kobe University, Japan


Article: Influence of functional and cross-linking monomers and the amount of template on the performance of molecularly imprinted polymers in binding assays.Ecevit Yilmaz, Klaus Mosbach & Karsten Haupt.Analytical Communications, (1999) 36 (5):167-170.

Article: The use of immobilized templates - a new approach in molecular imprinting.Ecevit Yilmaz, Karsten Haupt & Klaus Mosbach.Angewandte Chemie, International Edition in English, (2000) 39 (12): 2115-2118.

Article: Investigation of various supports materials for the imprinting of immobilized templates.Ecevit Yilmaz, Klaus Mosbach & Karsten Haupt.Manuscript (2002).

Article: Preparation of catalytic molecularly imprinted polymers using immobilized transition state analogues.Ecevit Yilmaz, Kristian Lettau, Deljana Werner, Axel Warsinke, Frieder Scheller, Burkhard Micheel, Karsten Haupt & Klaus Mosbach.Manuscript (2002).

Article: Towards the resolution of peptides on novel imprinted stationary phases.Ecevit Yilmaz, Per Möller, Domingo Sanchez, Karsten Haupt & Klaus Mosbach.Manuscript (2002).

Article: A facile method for preparing molecularly imprinted polymer spheres using spherical silica templates.Ecevit Yilmaz, Olof Ramström, Per Möller, Domingo Sanchez & Klaus Mosbach.Journal of Materials Chemistry, (2002) 12 (5): 1577-1581.

Article: Fabrication of molecularly imprinted polymer arrays by ink-jet printing.Maria N. Velasco-Garcia, David C. Cullen, Ecevit Yilmaz & Klaus Mosbach. Biosensors for Environmental Technology, (BIOSET) Newsletter, (2000) 7, 5-10.

Subject classification (UKÄ)

  • Biochemistry and Molecular Biology


  • immobilized template
  • molecularly imprinted polymer
  • composite material
  • polymerization method
  • heterogeneous imprinting
  • surface
  • morphology
  • Polymer technology
  • Polymerteknik
  • biopolymers


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