Research output per year
Research output per year
Z Söderlund, A Ibáñez-Fonseca, S Hajizadeh, J C Rodríguez-Cabello, J Liu, L Ye, E Tykesson, L Elowsson, G Westergren-Thorsson
Research output: Contribution to journal › Article › peer-review
Healthy regeneration of tissue relies on a well-orchestrated release of growth factors. Herein, we show the use of synthetic glycosaminoglycans for controlled binding and release of growth factors to induce a desired cellular response. First, we screened glycosaminoglycans with growth factors of interest to determine kon (association rate constant), koff (dissociation rate constant), and Kd (equilibrium rate constant). As proof-of-concept, we functionalized an elastin-like recombinamer (ELR) hydrogel with a synthetic glycosaminoglycan and immobilized fibroblast growth factor 2 (FGF2), demonstrating that human umbilical vein endothelial cells cultured on top of ELR hydrogel differentiated into tube-like structures. Taking this concept further, we developed a tunable macroporous ELR cryogel material, containing a synthetic glycosaminoglycan and FGF2 that showed increased blood vessel formation and reduced immune response compared to control when implanted in a subcutaneous mouse model. These results demonstrated the possibility for specific release of desired growth factors in/from a modular 3D scaffold in vitro and in vivo.
Original language | English |
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Article number | 1349 |
Journal | Communications Biology |
Volume | 5 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2022 Dec 8 |
Research output: Thesis › Doctoral Thesis (compilation)