Convergence in probiotic Lactobacillus gut-adaptive responses in humans and mice

Maria L. Marco; Maaike C. de Vries; Michiel Wels; Douwe Molenaar; Peter Mangell; Siv Ahrné; Willem M. de Vos; Elaine E. Vaughan; Michiel Kleerebezem

doi:10.1038/ismej.2010.61

Convergence in probiotic Lactobacillus gut-adaptive responses in humans and mice

Maria L. Marco, Maaike C. de Vries, Michiel Wels, Douwe Molenaar, Peter Mangell, Siv Ahrné, Willem M. de Vos, Elaine E. Vaughan, Michiel Kleerebezem

Research output: Contribution to journal › Article › peer-review

Abstract

Probiotic bacteria provide unique opportunities to study the global responses and molecular mechanisms underlying the effects of gut-associated microorganisms in the human digestive tract. In this study, we show by comparative transcriptome analysis using DNA microarrays that the established probiotic Lactobacillus plantarum 299v specifically adapts its metabolic capacity in the human intestine for carbohydrate acquisition and expression of exopolysaccharide and protein-aceous cell surface compounds. This report constitutes the first application of global gene expression profiling of a commensal microorganism in the human gut. A core L. plantarum transcriptome expressed in the mammalian intestine was also determined through comparisons of L. plantarum 299v activities in humans to those found for L. plantarum WCFS1 in germ-free mice. These results identify the niche-specific adaptations of a dietary microorganism to the intestinal ecosystem and provide novel targets for molecular analysis of microbial-host interactions which affect human health. The ISME Journal (2010) 4, 1481-1484; doi: 10.1038/ismej.2010.61; published online 27 May 2010

Original language	English
Pages (from-to)	1481-1484
Journal	The Isme Journal
Volume	4
Issue number	11
DOIs	https://doi.org/10.1038/ismej.2010.61
Publication status	Published - 2010

Bibliographical note

The information about affiliations in this record was updated in December 2015.
The record was previously connected to the following departments: Surgery Research Unit (013242220), Applied Nutrition and Food Chemistry (011001300)

Subject classification (UKÄ)

Surgery

Free keywords

intestine
transcript profiling
germ-free mice
gut microbiota
diet
in vivo-inducible genes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/ismej.2010.61

Cite this

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title = "Convergence in probiotic Lactobacillus gut-adaptive responses in humans and mice",

abstract = "Probiotic bacteria provide unique opportunities to study the global responses and molecular mechanisms underlying the effects of gut-associated microorganisms in the human digestive tract. In this study, we show by comparative transcriptome analysis using DNA microarrays that the established probiotic Lactobacillus plantarum 299v specifically adapts its metabolic capacity in the human intestine for carbohydrate acquisition and expression of exopolysaccharide and protein-aceous cell surface compounds. This report constitutes the first application of global gene expression profiling of a commensal microorganism in the human gut. A core L. plantarum transcriptome expressed in the mammalian intestine was also determined through comparisons of L. plantarum 299v activities in humans to those found for L. plantarum WCFS1 in germ-free mice. These results identify the niche-specific adaptations of a dietary microorganism to the intestinal ecosystem and provide novel targets for molecular analysis of microbial-host interactions which affect human health. The ISME Journal (2010) 4, 1481-1484; doi: 10.1038/ismej.2010.61; published online 27 May 2010",

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author = "Marco, {Maria L.} and {de Vries}, {Maaike C.} and Michiel Wels and Douwe Molenaar and Peter Mangell and Siv Ahrn{\'e} and {de Vos}, {Willem M.} and Vaughan, {Elaine E.} and Michiel Kleerebezem",

note = "The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Surgery Research Unit (013242220), Applied Nutrition and Food Chemistry (011001300)",

year = "2010",

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AU - de Vries, Maaike C.

AU - Wels, Michiel

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AU - Mangell, Peter

AU - Ahrné, Siv

AU - de Vos, Willem M.

AU - Vaughan, Elaine E.

AU - Kleerebezem, Michiel

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Surgery Research Unit (013242220), Applied Nutrition and Food Chemistry (011001300)

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N2 - Probiotic bacteria provide unique opportunities to study the global responses and molecular mechanisms underlying the effects of gut-associated microorganisms in the human digestive tract. In this study, we show by comparative transcriptome analysis using DNA microarrays that the established probiotic Lactobacillus plantarum 299v specifically adapts its metabolic capacity in the human intestine for carbohydrate acquisition and expression of exopolysaccharide and protein-aceous cell surface compounds. This report constitutes the first application of global gene expression profiling of a commensal microorganism in the human gut. A core L. plantarum transcriptome expressed in the mammalian intestine was also determined through comparisons of L. plantarum 299v activities in humans to those found for L. plantarum WCFS1 in germ-free mice. These results identify the niche-specific adaptations of a dietary microorganism to the intestinal ecosystem and provide novel targets for molecular analysis of microbial-host interactions which affect human health. The ISME Journal (2010) 4, 1481-1484; doi: 10.1038/ismej.2010.61; published online 27 May 2010

AB - Probiotic bacteria provide unique opportunities to study the global responses and molecular mechanisms underlying the effects of gut-associated microorganisms in the human digestive tract. In this study, we show by comparative transcriptome analysis using DNA microarrays that the established probiotic Lactobacillus plantarum 299v specifically adapts its metabolic capacity in the human intestine for carbohydrate acquisition and expression of exopolysaccharide and protein-aceous cell surface compounds. This report constitutes the first application of global gene expression profiling of a commensal microorganism in the human gut. A core L. plantarum transcriptome expressed in the mammalian intestine was also determined through comparisons of L. plantarum 299v activities in humans to those found for L. plantarum WCFS1 in germ-free mice. These results identify the niche-specific adaptations of a dietary microorganism to the intestinal ecosystem and provide novel targets for molecular analysis of microbial-host interactions which affect human health. The ISME Journal (2010) 4, 1481-1484; doi: 10.1038/ismej.2010.61; published online 27 May 2010

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Convergence in probiotic Lactobacillus gut-adaptive responses in humans and mice

Abstract

Bibliographical note

Subject classification (UKÄ)

Free keywords

UN SDGs

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Cite this