Research output per year
Research output per year
Björn Andersson, Karin Rengefors, Olga Kourtchenko, Kerstin Johannesson, Olof Berglund, Helena L. Filipsson
Research output: Contribution to journal › Article › peer-review
Resurrection studies can answer some fundamental questions in aquatic ecology and evolutionary biology. For phytoplankton resting stages, longevity of thousands to millions of years has recently been reported. However, contamination during sediment sampling could distort these estimates, and this risk has not been systematically evaluated. Here we used 4.5 μm diameter microspheres to quantify contamination while reviving the resting stages of seven abundant estuarine diatom and cyanobacterial taxa. We observed a sharp decline in resting stages abundance from 106 (g wet sediment)−1 at the surface to < 0.8 (g wet sediment)−1 at 12.5 cm depth. Added microspheres (~ 4.5 × 107 cm−2) were translocated even deeper down the sediment and could well explain the vertical distributions and abundances of revived cells. Without this control, we could have claimed to have revived seven multi-decades to centennial-old taxa. Our findings suggest that improved contamination controls are needed for sediment core sampling of rare cells, microfossils, or DNA molecules.
Original language | English |
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Pages (from-to) | 376-384 |
Journal | Limnology and Oceanography Letters |
Volume | 8 |
DOIs | |
Publication status | Published - 2022 |
Research output: Contribution to journal › Article › peer-review
Research output: Contribution to journal › Article › peer-review
Filipsson, H. (Researcher), Rengefors, K. (Researcher) & Berglund, O. (Researcher)
Stiftelsen Oscar och Lili Lamms Minne
Project: Research